Transcriptome-wide identification of WRKY transcription factors and their expression profiles under different stress in Cynanchum thesioides

被引：14

作者：

Chang, Xiaoyao ^{[1
]}

Yang, Zhongren ^{[1
]}

Zhang, Xiaoyan ^{[1
]}

Zhang, Fenglan ^{[1
]}

Huang, Xiumei ^{[1
]}

Han, Xu ^{[1
]}

机构：

[1] Inner Mongolia Agr Univ, Coll Hort & Plant Protect, Hohhot, Inner Mongolia, Peoples R China

来源：

PEERJ | 2022年 / 10卷

关键词：

WRKY gene family; Cynanchum thesioides; Plant hormones; Abiotic stresss; Gene expression; GENE FAMILY; ARABIDOPSIS; PROTEIN; TOLERANCE; OVEREXPRESSION; SUPERFAMILY; BINDING; GROWTH; RICE;

D O I：

10.7717/peerj.14436

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cynanchum thesioides (Freyn) K. Schum. is an important economic and medicinal plant widely distributed in northern China. WRKY transcription factors (TFs) play important roles in plant growth, development and regulating responses. However, there is no report on the WRKY genes in Cynanchum thesioides. A total of 19 WRKY transcriptome sequences with complete ORFs were identified as WRKY transcriptome sequences by searching for WRKYs in RNA sequencing data. Then, the WRKY genes were classified by phylogenetic and conserved motif analysis of the WRKY family in Cynanchum thesioides and Arabidopsis thaliana. qRT-PCR was used to determine the expression patterns of 19 CtWRKY genes in different tissues and seedlings of Cynanchum thesioides under plant hormone (ABA and ETH) and abiotic stresses (cold and salt). The results showed that 19 CtWRKY genes could be divided into groups I-III according to their structure and phylogenetic characteristics, and group II could be divided into five subgroups. The prediction of CtWRKY gene protein interactions indicates that CtWRKY is involved in many biological processes. In addition, the CtWRKY gene was differentially expressed in different tissues and positively responded to abiotic stress and phytohormone treatment, among which CtWRKY9, CtWRKY18, and CtWRKY19 were significantly induced under various stresses. This study is the first to identify the WRKY gene family in Cynanchum thesioides, and the systematic analysis lays a foundation for further identification of the function of WRKY genes in Cynanchum thesioides.

引用

页数：21

共 56 条

[1] Transcription factors WRKY11 and WRKY17 are involved in abiotic stress responses in Arabidopsis [J].

Ali, Muhammad Amjad ;

Azeem, Farrukh ;

Nawaz, Muhammad Amjad ;

Acet, Tuba ;

Abbas, Amjad ;

Imran, Qari Muhammad ;

Shah, Kausar Hussain ;

Rehman, Hafiz Mamoon ;

Chung, Gyuhwa ;

Yang, Seung Hwan ;

Bohlmann, Holger .

JOURNAL OF PLANT PHYSIOLOGY, 2018, 226 :12-21

[2] The MEME Suite [J].

Bailey, Timothy L. ;

Johnson, James ;

Grant, Charles E. ;

Noble, William S. .

NUCLEIC ACIDS RESEARCH, 2015, 43 (W1) :W39-W49

[3] WRKY transcription factors Jack of many trades in plants [J].

Bakshi, Madhunita ;

Oelmueller, Ralf .

PLANT SIGNALING & BEHAVIOR, 2014, 9 (02)

[4]

Bateman A, 2002, NUCLEIC ACIDS RES, V30, P276, DOI [10.1093/nar/gkp985, 10.1093/nar/gkr1065, 10.1093/nar/gkh121]

[5] Constitutively Active Mitogen-Activated Protein Kinase Versions Reveal Functions of Arabidopsis MPK4 in Pathogen Defense Signaling [J].

Berriri, Souha ;

Garcia, Ana Victoria ;

Frey, Nicolas Frei Dit ;

Rozhon, Wilfried ;

Pateyron, Stephanie ;

Leonhardt, Nathalie ;

Montillet, Jean-Luc ;

Leung, Jeffrey ;

Hirt, Heribert ;

Colcombet, Jean .

PLANT CELL, 2012, 24 (10) :4281-4293

[6] TBtools: An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data [J].

Chen, Chengjie ;

Chen, Hao ;

Zhang, Yi ;

Thomas, Hannah R. ;

Frank, Margaret H. ;

He, Yehua ;

Xia, Rui .

MOLECULAR PLANT, 2020, 13 (08) :1194-1202

[7] Genome-wide analysis of theWRKYgene family in the cucumber genome and transcriptome-wide identification of WRKY transcription factors that respond to biotic and abiotic stresses [J].

Chen, Chunhua ;

Chen, Xueqian ;

Han, Jing ;

Lu, Wenli ;

Ren, Zhonghai .

BMC PLANT BIOLOGY, 2020, 20 (01)

[8] Comparative Genomic Analysis Reveals Extensive Genetic Variations of WRKYs in Solanaceae and Functional Variations of CaWRKYs in Pepper [J].

Cheng, Yuan ;

Ahammed, Golam Jalal ;

Yao, Zhuping ;

Ye, Qingjing ;

Ruan, Meiying ;

Wang, Rongqing ;

Li, Zhimiao ;

Zhou, Guozhi ;

Wan, Hongjian .

FRONTIERS IN GENETICS, 2019, 10

[9] ProteinProtein Interactions in the Regulation of WRKY Transcription Factors [J].

Chi, Yingjun ;

Yang, Yan ;

Zhou, Yuan ;

Zhou, Jie ;

Fan, Baofang ;

Yu, Jing-Quan ;

Chen, Zhixiang .

MOLECULAR PLANT, 2013, 6 (02) :287-300

[10] Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response [J].

Dong, JX ;

Chen, CH ;

Chen, ZX .

PLANT MOLECULAR BIOLOGY, 2003, 51 (01) :21-37

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