Epigenomic profiling of cancer cells

被引：34

作者：

Gargiulo, Gaetano ^{[1
]}

Minucci, Saverio ^{[1
,2
]}

机构：

[1] European Inst Oncol, Dept Expt Oncol, I-20139 Milan, Italy

[2] Univ Milan, Dept Biomol Sci & Biotechnol, I-20133 Milan, Italy

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | 2009年 / 41卷 / 01期

关键词：

Cancer epigenomics; Next generation sequencing; DNA methylation; Histone post-translational modification; Chromatin accessibility; DNA METHYLATION PATTERNS; TUMOR-SUPPRESSOR GENES; EMBRYONIC STEM-CELLS; FACTOR-BINDING-SITES; GENOME-WIDE ANALYSIS; GROUP PROTEIN EZH2; HYPERSENSITIVE SITES; CHROMATIN-STRUCTURE; NONCODING RNAS; HISTONE MODIFICATIONS;

D O I：

10.1016/j.biocel.2008.07.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA methylation, post-translational modifications of histones and high order organization of chromatin in cell nuclei are the components of the epigenome. Epigenetic regulation of gene ne expression is specific for each Cell type, Within different tissues, according to stages of development and (in the adult organism) of differentiation. Almost invariably. this regulation is altered in disease states, including cancer. The complete Understanding of the identity of the epigenome of cancer has been so far hampered, due to the technical limitations and costs of the genome-wide analyses required. The recent development of next generation sequencing (NGS) technologies, however, holds the promise of fast, reliable and cost-effective analyses. Here we review the main approaches employed thus far to identify altered epigenetic patter its in cancer cells, and analyse how they are predicted to evolve in the scenario of the ultra high-throughput (UHT) screenings. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：127 / 135

页数：9

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