Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions

被引：77

作者：

Zhu, Dongjie ^{[1
,2
]}

Wang, Xiangxi ^{[1
]}

Fang, Qianglin ^{[3
]}

Van Etten, James L. ^{[4
,5
]}

Rossmann, Michael G. ^{[3
]}

Rao, Zihe ^{[1
,6
,7
]}

Zhang, Xinzheng ^{[1
,6
,8
]}

机构：

[1] Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing 100101, Peoples R China

[2] Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China

[3] Purdue Univ, Dept Biol Sci, 240 South Martin Jischke Dr, W Lafayette, IN 47907 USA

[4] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA

[5] Univ Nebraska, Nebraska Ctr Virol, Lincoln, NE 68583 USA

[6] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[7] Tsinghua Univ, Sch Med, Lab Struct Biol, Beijing 100084, Peoples R China

[8] Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Ctr Biol Imaging, Beijing 100101, Peoples R China

来源：

NATURE COMMUNICATIONS | 2018年 / 9卷

基金：

美国国家卫生研究院; 国家重点研发计划;

关键词：

ANGSTROM RESOLUTION; ELECTRON-MICROSCOPY; CRYOELECTRON MICROSCOPY; ATOMIC-STRUCTURE; CTF CORRECTION; VIRUSES; COMPLEX; CRYOMICROSCOPY; MICROGRAPHS; IMPROVES;

D O I：

10.1038/s41467-018-04051-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Ewald sphere effect is generally neglected when using the Central Projection Theorem for cryo electron microscopy single-particle reconstructions. This can reduce the resolution of a reconstruction. Here we estimate the attainable resolution and report a "block-based" reconstruction method for extending the resolution limit. We find the Ewald sphere effect limits the resolution of large objects, especially large viruses. After processing two real datasets of large viruses, we show that our procedure can extend the resolution for both datasets and can accommodate the flexibility associated with large protein complexes.

引用

页数：7

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