Optimization and enhanced production of α-amylase and protease by a newly isolated Bacillus licheniformis ZB-05 under solid-state fermentation

被引：27

作者：

Karatas, Hakan ^{[1
]}

Uyar, Fikret ^{[1
]}

Tolan, Veysel ^{[1
]}

Baysal, Zubeyde ^{[2
]}

机构：

[1] Dicle Univ, Dept Biol, TR-21280 Diyarbakir, Turkey

[2] Dicle Univ, Dept Chem, TR-21280 Diyarbakir, Turkey

来源：

ANNALS OF MICROBIOLOGY | 2013年 / 63卷 / 01期

关键词：

alpha-amylase; Agricultural waste; Bacillus licheniformis; Enzyme production; Protease; Solid-state fermentation; ALKALINE PROTEASE; SUBSTRATE FERMENTATION; PROCESS PARAMETERS; SUBTILIS; AMYLOLIQUEFACIENS; BIOPROCESSES; PURIFICATION; HYDROLYSIS; HUSK;

D O I：

10.1007/s13213-012-0443-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Eight different agro-residues were tested for alpha-amylase and protease production by using Bacillus licheniformis ZB-05. Among them, rice husk (RH) was proved as the best substrate for two enzymes (alpha-amylase 443 U/g and protease 469,000 U/g). Maximum enzyme production was observed to be 30 % initial moisture, with a growth period of 36 h in 20 and 30 % inoculum volumes for alpha-amylase and protease, respectively. The best enzyme recovery from solid mass was obtained when extracted with tap water. Among the tested various nitrogen sources, 1 % ammonium sulphate followed by 2 % Bacto liver, 2 % ammonium sulphate and 1 % Bacto casaminoacid served as the best inorganic and organic nitrogen sources for alpha-amylase and protease production, respectively. As additional carbon sources, 2 % soluble starch enhanced alpha-amylase production, while 1 % maltose enhanced protease production.

引用

页码：45 / 52

页数：8

共 35 条

[1] Alkaline protease production by a soil isolate of Beauveria felina under SSF condition:: parameter optimization and application to soy protein hydrolysis [J].