Photochemical inactivation of recombinant baculovirus

被引：4
作者：
Weightman, SA [1 ]
Banks, M [1 ]
机构：
[1] Vet Labs Agcy, Addlestone KT15 3NB, Surrey, England
来源：
JOURNAL OF VIROLOGICAL METHODS | 1999年 / 81卷 / 1-2期
关键词：
baculovirus; psoralen; photochemical inactivation; trioxalen; ELISA;
D O I：
10.1016/S0166-0934(99)00076-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method for the inactivation of aqueous suspensions of recombinant baculoviruses was developed using the combined effects of 4,5,8, trimethyl psoralen (trioxalen) and long-wave length UV illumination. A series of dose-effect titrations established the optimum conditions for the complete inactivation of a recombinant baculovirus expressing the glycoprotein D gene of Aujeszky's disease virus. The method was found to be equally effective against parental baculoviruses. The photochemical inactivation method proved simple, rapid and effective with minimal loss of biological activity in a recombinant protein subsequently used in an ELISA. Crown copyright (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：179 / 182
页数：4
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