Pathogenic Escherichia coli and Salmonella spp. in Chicken Carcass Rinses: Isolation and Genotyping by ERIC-PCR

被引:13
作者
Telli, Arife Ezgi [1 ]
Bicer, Yusuf [1 ]
Telli, Nihat [2 ]
Gungor, Candan [3 ]
Turkal, Gamze [1 ]
Onmaz, Nurhan Ertas [3 ]
机构
[1] Selcuk Univ, Fac Vet Med, Dept Food Hyg & Technol, TR-42130 Konya, Turkey
[2] Konya Tech Univ, Vocat Sch Tech Sci, Dept Food Proc, TR-42250 Konya, Turkey
[3] Erciyes Univ, Fac Vet Med, Dept Food Hyg & Technol, TR-38280 Kayseri, Turkey
关键词
Escherichia coli; Chicken carcass; Salmonella spp; ERIC-PCR; Genotyping; POLYMERASE-CHAIN-REACTION; ANTIMICROBIAL-RESISTANCE; BROILER-CHICKENS; RAPID DETECTION; TYPHIMURIUM; POULTRY; STRAINS; IDENTIFICATION; ENTERITIDIS; PROFILES;
D O I
10.29261/pakvetj/2022.049
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The present study aimed to determine the pathogenic Escherichia coli and Salmonella spp. and to investigate their phylogenetic relation by Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) in retail chicken samples. A total of 75 samples were processed for isolation of E. coli and Salmonella spp. by classical cultural methods and isolates were confirmed by the species-specific PCR. Salmonella spp. was detected in 21.3% and E. coli was detected in 74.6% of the chicken carcasses. S. Enteritidis and S. Typhimurium were not detected in chickens by duplex PCR-based assay. O157 based on serotyping and PCR, was not detected in any of the isolates. Besides, virulence and toxin genes were not detected in any of the E. coli isolates. According to ERIC patterns, the obtained ribotypes showed that all Salmonella spp. isolates presented large genetic diversity, whereas only two (3.5%) of E. coli isolates were genetically identical. Although virulent E. coli, and pathogenic serotypes of Salmonella spp. were not detected in our study, it is thought that their high incidence should be considered as an indicator of failure to comply with hygienic conditions and lack of sanitary practices especially in slaughterhouses.
引用
收藏
页码:493 / 498
页数:6
相关论文
共 49 条
[1]  
Adesiji Y., 2011, Sierra Leone J. Biomed. Res, V3, P8, DOI [10.4314/sljbr.v3i1.66644, DOI 10.4314/SLJBR.V3I1.66644]
[2]  
[Anonymous], 2014, J ANTI-INFECT
[3]  
Apan TZ., 2018, KIRIKKALE UNITIP DER, V20, P256
[4]  
Ardakani Maryam Afkhami, 2016, Electron Physician, V8, P2291, DOI 10.19082/2291
[5]   Detection of Escherichia coli and Salmonella in chicken rinse carcasses [J].
Asensi, G. F. ;
dos Reis, E. M. F. ;
Del Aguila, E. M. ;
Rodrigues, D. dos P. ;
Silva, J. T. ;
Paschoalin, V. M. F. .
BRITISH FOOD JOURNAL, 2009, 111 (6-7) :517-527
[6]  
Balakrishnan S., 2018, Journal of Entomology and Zoology studies, V6, P2468
[7]   Enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis as a reliable evidence for suspected Shigella spp. outbreaks [J].
Balzhshi, Bita ;
Afshari, Nasim ;
Fallah, Fatemeh .
BRAZILIAN JOURNAL OF MICROBIOLOGY, 2018, 49 (03) :529-533
[8]   A diagnostic one-step real-time reverse transcription polymerase chain reaction method for accurate detection of influenza virus type A [J].
Behzadi, Mohammad Amin ;
Ziyaeyan, Mazyar ;
Alborzi, Abdolvahab .
ARCHIVES OF MEDICAL SCIENCE, 2016, 12 (06) :1286-1292
[9]   ERIC-PCR technique applied to monitoring and quantification of DNA damage during water disinfection process [J].
Cristiani, Mariana ;
Flores, Marina J. ;
Brandi, Rodolfo J. ;
Tedeschi, Fabian A. ;
Zalazar, Fabian E. ;
Labas, Marisol D. .
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2020, 202
[10]   Genetic variability of avian Escherichia coli strains evaluated. by enterobacterial repetitive intergenic consensus and repetitive extragenic palindromic polymerase chain reaction [J].
de Moura, AC ;
Irino, K ;
Vidotto, MC .
AVIAN DISEASES, 2001, 45 (01) :173-181