Characterization of pbpA and pbp2 encoding penicillin-binding proteins located on the downstream of clavulanic acid gene cluster in Streptomyces clavuligerus

被引：16
作者：
Ishida, K
Hung, TV
Liou, K
Lee, HC
Shin, CH
Sohng, JK
机构：
[1] Sun Moon Univ, Inst Biomol Reconstruct, Asan 336708, Chungnam, South Korea
[2] CDK BIO Res Inst, Asan, Kyunggi, South Korea
来源：
BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 06期
关键词：
actinomycetes; clavulanic acid; penicillin-binding protein; Streptomyces clavuligerus;
D O I：
10.1007/s10529-005-6071-5
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Two genes, pbpA (orf18) and pbp2 (orf19) located on the downstream of clavulanic acid (CA) gene cluster of Streptomyces clavuligerus were cloned into pET-28a(+), and confirmed to encode a family of high molecular-weight penicillin-binding proteins (PBPs). Both genes were amplified from genomic DNA by PCR and expressed in E. coli BL21 (DE3). Hydropathy plots of the proteins revealed a single stretch of hydrophobic amino acids indicating them to be transmembrane proteins. Pbp2 had lower affinity to penicillin G compared to PbpA, and was essential to the cell growth in contrast to PbpA.
引用
收藏
页码：409 / 417
页数：9
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