Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals

被引：16

作者：

Awel, Salah ^{[1
,2
]}

Kirian, Richard A. ^{[3
]}

Wiedorn, Max O. ^{[1
,4
]}

Beyerlein, Kenneth R. ^{[1
]}

Roth, Nils ^{[1
]}

Horke, Daniel A. ^{[1
,2
]}

Oberthuer, Dominik ^{[1
]}

Knoska, Juraj ^{[1
,4
]}

Mariani, Valerio ^{[1
]}

Morgan, Andrew ^{[1
]}

Adriano, Luigi ^{[5
]}

Tolstikova, Alexandra ^{[1
,4
]}

Xavier, P. Lourdu ^{[1
,6
]}

Yefanov, Oleksandr ^{[1
]}

Aquila, Andrew ^{[7
]}

Barty, Anton ^{[1
]}

Roy-Chowdhury, Shatabdi ^{[3
]}

Hunter, Mark S. ^{[7
]}

James, Daniel ^{[3
]}

Robinson, Joseph S. ^{[7
]}

Weierstall, Uwe ^{[3
]}

Rode, Andrei V. ^{[8
]}

Bajt, Sasa ^{[5
]}

Kuepper, Jochen ^{[1
,2
,4
]}

Chapman, Henry N. ^{[1
,2
,4
]}

机构：

[1] Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, Notkestr 85, D-22607 Hamburg, Germany

[2] Univ Hamburg, Hamburg Ctr Ultrafast Imaging, Luruper Chaussee 149, D-22761 Hamburg, Germany

[3] Arizona State Univ, Tempe, AZ USA

[4] Univ Hamburg, Dept Phys, Luruper Chaussee 149, D-22761 Hamburg, Germany

[5] Deutsch Elektronen Synchrotron DESY, Photon Sci, Notkestr 85, D-22607 Hamburg, Germany

[6] Max Planck Inst Struct & Dynam Matter, Luruper Chaussee 149, D-22761 Hamburg, Germany

[7] SLAC Natl Accelerator Lab, Linac Coherent Light Source LCLS, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA

[8] Australian Natl Univ, Res Sch Phys & Engn, Laser Phys Ctr, Canberra, ACT 2601, Australia

来源：

JOURNAL OF APPLIED CRYSTALLOGRAPHY | 2018年 / 51卷

基金：

澳大利亚研究理事会; 欧洲研究理事会;

关键词：

X-ray diffraction; aerosols; nanocrystals; FREE-ELECTRON LASER; CRYSTALLOGRAPHY; CRYSTALS;

D O I：

10.1107/S1600576717018131

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.

引用

页码：133 / 139

页数：7

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