Single-cell transcriptomics reveals gene expression dynamics of human fetal kidney development

被引：103

作者：

Hochane, Mazene ^{[1
]}

van den Berg, Patrick R. ^{[1
]}

Fan, Xueying ^{[2
]}

Berenger-Curries, Noemie ^{[1
]}

Adegeest, Esmee ^{[1
]}

Bialecka, Monika ^{[2
]}

Nieveen, Maaike ^{[2
]}

Menschaart, Maarten ^{[1
]}

Lopes, Susana M. Chuva de Sousa ^{[2
,3
]}

Semrau, Stefan ^{[1
]}

机构：

[1] Leiden Univ, Leiden Inst Phys, Leiden, Netherlands

[2] Leiden Univ, Dept Anat & Embryol, Med Ctr, Leiden, Netherlands

[3] Ghent Univ Hosp, Dept Reprod Med, Ghent, Belgium

来源：

PLOS BIOLOGY | 2019年 / 17卷 / 02期

基金：

欧洲研究理事会;

关键词：

BRANCHING MORPHOGENESIS; PROTEIN NEPHRONECTIN; BASEMENT-MEMBRANE; BINDING PROTEIN; PROGENITORS; MIDKINE; MOUSE; DIFFERENTIATION; IDENTIFICATION; SEGMENTATION;

D O I：

10.1371/journal.pbio.3000152

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The current understanding of mammalian kidney development is largely based on mouse models. Recent landmark studies revealed pervasive differences in renal embryogenesis between mouse and human. The scarcity of detailed gene expression data in humans therefore hampers a thorough understanding of human kidney development and the possible developmental origin of kidney diseases. In this paper, we present a single-cell transcriptomics study of the human fetal kidney. We identified 22 cell types and a host of marker genes. Comparison of samples from different developmental ages revealed continuous gene expression changes in podocytes. To demonstrate the usefulness of our data set, we explored the heterogeneity of the nephrogenic niche, localized podocyte precursors, and confirmed disease-associated marker genes. With close to 18,000 renal cells from five different developmental ages, this study provides a rich resource for the elucidation of human kidney development, easily accessible through an interactive web application.

引用

页数：31

共 73 条

[1] Psychrophilic proteases dramatically reduce single-cell RNA-seq artifacts: a molecular atlas of kidney development [J].