Bone regeneration induced by a 3D architectured hydrogel in a rat critical-size calvarial defect

被引：65

作者：

Lohmann, P. ^{[1
]}

Willuweit, A. ^{[1
]}

Neffe, A. T. ^{[2
,3
]}

Geisler, S. ^{[1
]}

Gebauer, T. P. ^{[2
,3
]}

Beer, S. ^{[4
]}

Coenen, H. H. ^{[5
]}

Fischer, H. ^{[6
]}

Hermanns-Sachweh, B. ^{[7
]}

Lendlein, A. ^{[2
,3
]}

Shah, N. J. ^{[1
,8
,9
,10
]}

Kiessling, F. ^{[11
,12
]}

Langen, K. - J. ^{[1
,13
]}

机构：

[1] Forschungszentrum Julich, Inst Neurosci & Med INM 4, Wilhelm Johnen Str, D-52428 Julich, Germany

[2] Helmholtz Zentrum Geesthacht, Inst Biomat Sci, Kantstr 55, D-14513 Teltow, Germany

[3] Helmholtz Zentrum Geesthacht, Berlin Brandenburg Ctr Regenerat Therapies, Kantstr 55, D-14513 Teltow, Germany

[4] Forschungszentrum Julich, Inst Neurosci & Med INM 2, Wilhelm Johnen Str, D-52428 Julich, Germany

[5] Forschungszentrum Julich, Inst Neurosci & Med INM 5, Wilhelm Johnen Str, D-52428 Julich, Germany

[6] RWTH Aachen Univ Hosp, Dept Dent Mat & Biomat Res, Pauwelsstr 30, D-52074 Aachen, Germany

[7] RWTH Aachen Univ Hosp, Dept Pathol, Pauwelsstr 30, D-52074 Aachen, Germany

[8] Forschungszentrum Julich, Inst Neurosci & Med INM 11, Wilhelm Johnen Str, D-52428 Julich, Germany

[9] Monash Univ, Dept Elect & Comp Syst Engn, Melbourne, Vic, Australia

[10] Monash Univ, Monash Biomed Imaging, Sch Psychol Sci, Melbourne, Vic, Australia

[11] Rhein Westfal TH Aachen, Inst Expt Mol Imaging, Univ Clin, Pauwelsstr 30, D-52074 Aachen, Germany

[12] Rhein Westfal TH Aachen, Helmholtz Inst Biomed Engn, Pauwelsstr 30, D-52074 Aachen, Germany

[13] RWTH Aachen Univ Hosp, Dept Nucl Med, Pauwelsstr 30, D-52074 Aachen, Germany

来源：

BIOMATERIALS | 2017年 / 113卷

关键词：

Material-induced bone regeneration; Regenerative medicine; Critical-size calvarial defect; Positron-emission-tomography (PET); Micro-computed tomography (mu-CT); Push-out test; F-18-FDG PET; IN-VIVO; COLLAGEN; PRESERVATION; STRATEGIES; SOFTWARE; REPAIR; GRAFT;

D O I：

10.1016/j.biomaterials.2016.10.039

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Bone regeneration can be stimulated by implantation of biomaterials, which is especially important for larger bone defects. Here, healing potency of the porous ArcGel was evaluated in a critical-size calvarial bone defect in rats in comparison with clinical standard autologous bone and Bio-Oss (R) Collagen (BioOss), a bone graft material frequently used in clinics. Bone healing and metabolic processes involved were monitored longitudinally by [(18)]-fluoride and [F-18]-FDG mu-PET/CT 1d, 3d, 3w, 6w, and 12w post implantation. Differences in quality of bone healing were assessed by ex vivo mu-CT, mechanical tests and histomorphometry. The amount of bone formed after implantation of ArcGel was comparable to autologous bone and superior to BioOss (histomorphometry). Furthermore, microarchitecture of newly formed bone was more physiological and better functional in case of ArcGel (push-out tests). [F-18]-FDG uptake increased until 3d after implantation, and decreased until 12w for both ArcGel and BioOss. [F-18]-fluoride uptake increased until 3w post implantation for all materials, but persisted significantly longer at higher levels for BioOss, which indicates a prolonged remodelling phase. The study demonstrates the potential of ArcGel to induce restitutio ad integrum comparable with clinical standard autologous bone and better bone regeneration in large defects compared to a commercial state-of-the-art biomaterial. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：158 / 169

页数：12

共 48 条

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