Quantification of Plasma Cell-Free Circulating DNA at Different Stages of Colorectal Cancer

被引：24
作者：
Agah, Shahram [1 ]
Akbari, Abolfazl [1 ]
Talebi, Atefeh [1 ]
Masoudi, Mohsen [1 ]
Sarveazad, Arash [1 ]
Mirzaei, Alireza [2 ]
Nazmi, Farinaz [3 ]
机构：
[1] Iran Univ Med Sci, Colorectal Res Ctr, Tehran, Iran
[2] Iran Univ Med Sci, Shafa Orthoped Hosp, Bone & Joint Reconstruct Res Ctr, Tehran, Iran
[3] Univ Tabriz, Fac Nat Sci, Dept Zool, Tabriz, Iran
来源：
CANCER INVESTIGATION | 2017年 / 35卷 / 10期
关键词：
Biomarker; Blood; Circulating DNA; Colorectal cancer; Plasma; LUNG-CANCER; NUCLEIC-ACIDS; TUMOR DNA; HEPATOCELLULAR-CARCINOMA; MICROSATELLITE ANALYSIS; QUANTITATIVE-ANALYSIS; BLOOD; SERUM; MARKER; DISEASE;
D O I：
10.1080/07357907.2017.1408814
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Cell-free circulating DNAs (cfcDNAs) have been recognized as promising biomarkers for a number of cancers. This study aimed to quantify the cfcDNA in colorectal cancer to assess its potential value as biomarker. Quantification of baseline cfcDNA was determined as the amount of free glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in plasma, using quantitative real-time polymerase chain reaction (PCR). The calculated area under the curve (AUC) of receiver operating characteristic (ROC) for cfcDNA was 0.875 (95% CI, 0.811-0.94), which was indicative of a high discriminatory power (p<0.001) and significant accuracy in distinguishing cancer patients from healthy individuals. The quantification of cfcDNA could be useful for clinical settings of CRC.
引用
收藏
页码：625 / 632
页数：8
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