Inhibitory effect of high-dosage zinc oxide dietary supplementation on Campylobacter coli excretion in weaned piglets

被引：28

作者：

Bratz, K. ^{[1
]}

Goelz, G. ^{[1
]}

Riedel, C. ^{[1
]}

Janczyk, P. ^{[2
]}

Noeckler, K. ^{[2
]}

Alter, T. ^{[1
]}

机构：

[1] Free Univ Berlin, Inst Food Hyg, D-14163 Berlin, Germany

[2] Fed Inst Risk Assessment, Berlin, Germany

来源：

JOURNAL OF APPLIED MICROBIOLOGY | 2013年 / 115卷 / 05期

关键词：

Campylobacter coli; excretion; feed supplementation; pigs; zinc oxide;

D O I：

10.1111/jam.12307

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

AimsThis study investigated the impact of zinc oxide (ZnO) on Campylobacter coli by in vivo and in vitro assays. Methods and ResultsBy in vitro growth inhibition assays, a high susceptibility of Camp.coli against ZnO could be observed. At concentrations 26mmoll(-1) ZnO, a decline in cell numbers occurred. Quantitative real-time PCR assays demonstrated an up-regulation of the main oxidative stress gene (katA) in response to ZnO treatment. The expression level of katA was increased by fivefold after ZnO treatment. An experiment was carried out in pigs to elucidate the impact of ZnO as feed supplement on Camp.coli faecal excretion. Feeding a high-dosage ZnO concentration (3100mgkg(-1)) to piglets significantly reduced the faecal excretion of Camp.coli by up to 1logCFUg(-1) as compared to animals receiving a low (40mgkg(-1)) or medium (100mgkg(-1)) ZnO diet. ConclusionIn vitro assays showed a high susceptibility of Camp.coli against ZnO. Adding high levels of ZnO to the diet of weaned piglets reduced Camp.coli excretion significantly. There is evidence for the induction of an oxidative stress response by ZnO supplementation in Camp.coli. Significance and Impact of the StudySupplementation of a high-dosage ZnO diet to piglets can reduce the Camp.coli load, potentially leading to a lower contamination risk of meat during slaughter.

引用

页码：1194 / 1202

页数：9

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