Purification and identification of soluble Catechol-O-methyltransferase from rat liver

被引：0
作者：
Yang, Lina [1 ,2 ]
Yan, Jianhui [2 ]
Deng, Yulin [1 ]
机构：
[1] Beijing Inst Technol, Sch Life Sci & Technol, Beijing, Peoples R China
[2] Yiling Pharmaceut Grp, Beijing 100081, Peoples R China
来源：
2010 4TH INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING (ICBBE 2010) | 2010年
关键词：
Solublecatechol-O-methyltransferase; Offgel isoelectric focusing; purification; mass spectrometry; identification; GENE POLYMORPHISM; SCHIZOPHRENIA; ASSOCIATION;
D O I：
暂无
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Soluble catechol-O-methyltransferase (S-COMT) is an important target protein due to its involvement in some human disorders. The main scope of the work was to develop an approach to purify and identify S-COMT. Its isoelectric point was 5.0 determined with Offgel. A simplified process including extraction, selective precipitation, ammonium sulfate precipitation, gel filtration chromatography and anion exchange chromatography was established to obtain S-COMT of a certain purity degree. The molecular was 25kD. Mass spectrometry and mascot were employed for further identification.
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页数：4
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