Detection of Heteroresistant Mycobacterium tuberculosis by Pyrosequencing

被引：10

作者：

Engstrom, Anna ^{[1
,2
]}

Hoffner, Sven ^{[1
,2
]}

Jureen, Pontus ^{[1
,2
]}

机构：

[1] Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden

[2] Swedish Inst Communicable Dis Control, Dept Preparedness, Solna, Sweden

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2013年 / 51卷 / 12期

关键词：

RIFAMPIN RESISTANCE; RAPID DETECTION; ASSAY; STRAINS;

D O I：

10.1128/JCM.01761-13

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The ability of pyrosequencing to detect a resistant minority population of a heteroresistant Mycobacterium tuberculosis strain was investigated by performing a titration study. A mutant signal was noted only when the proportion of mutant DNA in the DNA target was 35 to 50%, showing that the sensitivity is significantly lower than that of phenotypic drug susceptibility test methods.

引用

页码：4210 / 4212

页数：3

共 17 条

[1]

Bigger JW, 1944, LANCET, V2, P497

[2] Molecular Detection of Mutations Associated with First- and Second-Line Drug Resistance Compared with Conventional Drug Susceptibility Testing of Mycobacterium tuberculosis [J].

Campbell, Patricia J. ;

Morlock, Glenn P. ;

Sikes, R. David ;

Dalton, Tracy L. ;

Metchock, Beverly ;

Starks, Angela M. ;

Hooks, Delaina P. ;

Cowan, Lauren S. ;

Plikaytis, Bonnie B. ;

Posey, James E. .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2011, 55 (05) :2032-2041

[3] Rapid, High-Throughput Detection of Rifampin Resistance and Heteroresistance in Mycobacterium tuberculosis by Use of Sloppy Molecular Beacon Melting Temperature Coding [J].

Chakravorty, Soumitesh ;

Kothari, Harsheel ;

Aladegbami, Bola ;

Cho, Eun Jin ;

Lee, Jong Seok ;

Roh, Sandy S. ;

Kim, Hyunchul ;

Kwak, Hyungkyung ;

Lee, Eun Gae ;

Hwang, Soo Hee ;

Banada, Padmapriya P. ;

Safi, Hassan ;

Via, Laura E. ;

Cho, Sang-Nae ;

Barry, Clifton E., III ;

Alland, David .

JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (07) :2194-2202

[4] Detection of First- and Second-Line Drug Resistance in Mycobacterium tuberculosis Clinical Isolates by Pyrosequencing [J].

Engstrom, Anna ;

Morcillo, Nora ;

Imperiale, Belen ;

Hoffner, Sven E. ;

Jureen, Pontus .

JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (06) :2026-2033

[5] Can Molecular Methods Detect 1% Isoniazid Resistance in Mycobacterium tuberculosis? [J].

Folkvardsen, Dorte Bek ;

Svensson, Erik ;

Thomsen, Vibeke O. ;

Rasmussen, Erik Michael ;

Bang, Didi ;

Werngren, Jim ;

Hoffner, Sven ;

Hillemann, Doris ;

Rigouts, Leen .

JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (05) :1596-1599

[6] Rapid Detection of Mycobacterium tuberculosis and Rifampin Resistance by Use of On-Demand, Near-Patient Technology [J].

Helb, Danica ;

Jones, Martin ;

Story, Elizabeth ;

Boehme, Catharina ;

Wallace, Ellen ;

Ho, Ken ;

Kop, JoAnn ;

Owens, Michelle R. ;

Rodgers, Richard ;

Banada, Padmapriya ;

Safi, Hassan ;

Blakemore, Robert ;

Lan, N. T. Ngoc ;

Jones-Lopez, Edward C. ;

Levi, Michael ;

Burday, Michele ;

Ayakaka, Irene ;

Mugerwa, Roy D. ;

McMillan, Bill ;

Winn-Deen, Emily ;

Christel, Lee ;

Dailey, Peter ;

Perkins, Mark D. ;

Persing, David H. ;

Alland, David .

JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (01) :229-237

[7] Evaluation of the GenoType MTBDRplus assay for rifampin and isoniazid susceptibility testing of Mycobacterium tuberculosis strains and clinical specimens [J].

Hillemann, Doris ;

Ruesch-Gerdes, Sabine ;

Richter, Elvira .

JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (08) :2635-2640

[8] Feasibility of the GenoType MTBDRsl Assay for Fluoroquinolone, Amikacin-Capreomycin, and Ethambutol Resistance Testing of Mycobacterium tuberculosis Strains and Clinical Specimens [J].

Hillemann, Doris ;

Ruesch-Gerdes, Sabine ;

Richter, Elvira .

JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (06) :1767-1772

[9] Mechanisms of heteroresistance to isoniazid and rifampin of Mycobacterium tuberculosis in Tashkent, Uzbekistan [J].

Hofmann-Thiel, S. ;

van Ingen, J. ;

Feldmann, K. ;

Turaev, L. ;

Uzakova, G. T. ;

Murmusaeva, G. ;

van Soolingen, D. ;

Hoffmann, H. .

EUROPEAN RESPIRATORY JOURNAL, 2009, 33 (02) :368-374

[10] Rapid detection of rifampin resistance in Mycobacterium tuberculosis by Pyrosequencing technology [J].

Jureen, Pontus ;

Engstrand, Lars ;

Eriksson, Solveig ;

Alderborn, Anders ;

Krabbe, Margareta ;

Hoffner, Sven E. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (06) :1925-1929

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