A Neurotoxic Secretory Phospholipase A2 Induces Apoptosis in Motoneuron-like Cells

被引：8
|
作者：
Praznikar, Zala Jenko [1 ]
Petan, Toni [1 ]
Pungercar, Joze [1 ]
机构：
[1] Jozef Stefan Inst, Dept Mol & Biomed Sci, SI-1000 Ljubljana, Slovenia
来源：
MECHANISMS OF EXOCYTOSIS | 2009年 / 1152卷
关键词：
motoneuronal cells; phospholipase A(2); ammodytoxin A; cytotoxicity; apoptosis; CEREBELLAR GRANULE NEURONS; BETA-BUNGAROTOXIN; RECEPTOR; DEATH; VENOM; ATHEROGENESIS; CYTOTOXICITY; ACTIVATION; EXPRESSION; MECHANISM;
D O I：
10.1111/j.1749-6632.2008.03999.x
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Ammodytoxin A (AtxA) is a presynaptically neurotoxic secretory phospholipase A(2) from snake venom. The aim of this study was to investigate the mechanism of its cytotoxicity expressed against mouse motoneuronal NSC34 cells. AtxA displayed a potent dose- and time-dependent cytotoxicity that was associated with apoptosis and not necrosis, as revealed by a reduction of mitochondrial membrane potential, activation of caspase-3, and by the absence of propidium iodide staining. The cytotoxic- and apoptosis-inducing effects of AtxA were specific for the motoneuronal cells; human embryonic kidney (HEK293) and mouse myoblast (C2C12) cells were shown to be resistant to the toxin.
引用
收藏
页码：215 / 224
页数：10
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