DEVELOPMENT OF MICROSATELLITE MARKERS IN ILEX KAUSHUE (AQUIFOLIACEAE), A MEDICINAL PLANT SPECIES

被引：1
|
作者：
Qin, Lan-Fang [1 ]
Qu, Xin-Cheng [1 ]
Hu, Gang [2 ]
Huang, Yun-Feng [1 ]
Zhang, Qi-Wei [1 ]
机构：
[1] Guangxi Inst Tradit Med & Pharmaceut Sci, Naning 530022, Peoples R China
[2] Guangxi Teachers Educ Univ, Sch Environm & Life Sci, Naning 530001, Peoples R China
来源：
APPLICATIONS IN PLANT SCIENCES | 2015年 / 3卷 / 08期
关键词：
Aquifoliaceae; genetic diversity; Ilex kaushue; microsatellite marker;
D O I：
10.3732/apps.1500040
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Premise of the study: Microsatellite markers were developed for Ilex kaushue (Aquifoliaceae), a medicinal plant with extremely small wild populations that exists in fragmented habitats, to assess and protect its genetic diversity. Methods and Results: Using 454 GS FLX Titanium sequencing, 16 microsatellite primer sets were isolated and characterized. Fifteen of these markers were polymorphic. The number of alleles per locus ranged from one to nine across 22 individuals from both cultivated and wild populations. The observed and expected heterozygosity in these two populations ranged from 0.000 to 1.000 and from 0.000 to 0.785, respectively. Conclusions: These markers will be useful in studies on genetic diversity of I. kaushue
引用
收藏
页数：3
相关论文
共 50 条
  • [21] A cryopreservation protocol for immature zygotic embryos of species of Ilex (Aquifoliaceae)
    Mroginski, Luis A.
    Sansberro, Pedro A.
    Scocchi, Adriana M.
    Luna, Claudia
    Rey, Hebe Y.
    BIOCELL, 2008, 32 (01) : 33 - 39
  • [22] A NEW SPECIES OF ILEX (AQUIFOLIACEAE) FROM CENTRAL-AMERICA
    HAHN, WJ
    ANNALS OF THE MISSOURI BOTANICAL GARDEN, 1988, 75 (02) : 733 - 735
  • [23] Ilex pubifructa (Aquifoliaceae), a new species from northern Thailand
    Pruesapan K.
    Andrews S.
    Simpson D.A.
    Kew Bulletin, 2017, 72 (2)
  • [24] Ilex pubifructa (Aquifoliaceae), a new species from northern Thailand
    Pruesapan, Kanchana
    Andrews, Susyn
    Simpson, David A.
    KEW BULLETIN, 2017, 72 (02)
  • [25] Development of 12 microsatellite markers for Aconitum brachypodum (Ranunculaceae), a critically endangered and endemic medicinal plant
    Li, Ya-Qiong
    Meng, Yi
    Zhang, Jie
    BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2015, 61 : 462 - 464
  • [26] Development and characterization of microsatellite markers in Sarracenia L. (pitcher plant) species
    Willie L. Rogers
    Jennifer M. Cruse-Sanders
    Ron Determann
    Russell L. Malmberg
    Conservation Genetics Resources, 2010, 2 : 75 - 79
  • [27] Development and characterization of microsatellite DNA markers for the Alpine plant species Campanula thyrsoides
    Aegisdottir, H. H.
    Koller, B.
    Kuss, P.
    Stoecklin, J.
    MOLECULAR ECOLOGY NOTES, 2007, 7 (06): : 996 - 997
  • [28] Development and characterization of microsatellite markers in Sarracenia L. (pitcher plant) species
    Rogers, Willie L.
    Cruse-Sanders, Jennifer M.
    Determann, Ron
    Malmberg, Russell L.
    CONSERVATION GENETICS RESOURCES, 2010, 2 (01) : 75 - 79
  • [29] Plant regeneration of Ilex paraguariensis (Aquifoliaceae) by in vitro culture of nodal segments
    Sansberro, P
    Rey, H
    Bernardis, A
    Luna, C
    Collavino, M
    Mroginski, L
    BIOCELL, 2000, 24 (01) : 53 - 63
  • [30] A new species of Ilex (Aquifoliaceae) from espinhaco range, bahia, brazil
    Groppo, Milton
    BOTANICAL JOURNAL OF THE LINNEAN SOCIETY, 2007, 155 (01) : 153 - 156
12345