Ultrasound-Triggered Gas-Generating Doxorubicin Poly(lactic-co-glycolic acid)-Nanoparticles for Cancer Therapy

The purpose of current research is to develop ultrasound-triggered gas-generating Doxorubicin PLGA nanoparticle for cancer therapy. Method: pH-sensitive PLGA nanoparticles (PLGANPs) was fabricated to deliver doxorubicin (DOX) and sodium bicarbonate (NaHCO3) using the water-in-oilin-water (w/o/w) double emulsion method. Result: The nanoparticle with the size (650 nm) and high drug loading (15.8 +/- 2.3%) were successfully prepared and showed pH-responsive release characteristics. In vitro results indicate that DOX/NaHCO3@PLGANPs with ultrasound had higher inhibition and cell uptake on MCF-7 cells than free DOX and other formulation. In vivo animal experiments showed that after treatment of DOX/NaHCO3@ PLGANPs with ultrasound, the relative tumor volume (0.63) of S180-tumor-bearing mice was lower than, that of without ultrasound (0.81), DOXO PLGANPs (1.00) and Free DOX (1.12), Moreover, safety evaluation result indicated that DOX/NaHCO(3)OPLGANPs was safer than free DOX. In conclusion, the DOX/NaHCO(3)OPLGANPs was successfully developed and evaluated in vitro and in vivo. This drug delivery system will be a promising strategy for cancer therapy and diagnosis.