A Comparison of Ypet and Firefly Luciferase as Reporter Proteins for High-Throughput Screening

被引：4
作者：
Yoon, Sei Mee [1 ,2 ]
Namkung, Wan [1 ,2 ]
Lee, Jinu [1 ]
机构：
[1] Yonsei Univ, Yonsei Inst Pharmaceut Sci, Coll Pharm, Inchon 406840, South Korea
[2] Yonsei Univ, Dept Integrated OMICS Biomed Sci, Seoul 120749, South Korea
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2013年 / 77卷 / 11期
基金：
新加坡国家研究基金会;
关键词：
Ypet; reporter protein; high-throughput screening; YELLOW FLUORESCENT PROTEIN; VARIANT; EXPRESSION; FRET;
D O I：
10.1271/bbb.130537
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
When Ypet was used as a reporter protein for high-throughput screening (HTS), it showed peak fold induction and a dynamic range similar to those for firefly luciferase. We also determined that conducting a reading immediately after media aspiration was the best method for HTS. We conclude that Ypet can serve as a substitute for luciferase as a reporter protein in HTS assays.
引用
收藏
页码：2328 / 2330
页数：3
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