Halobacterium salinarum storage and rehydration after spray drying and optimization of the processes for preservation of carotenoids

被引：0

作者：

Kalenov, Sergei V. ^{[1
]}

Gordienko, Mariia G. ^{[2
]}

Murzina, Ekaterina D. ^{[1
]}

Poberezhniy, Daniil Y. ^{[1
]}

Baurin, Dmitry V. ^{[1
]}

Suzina, Natalia E. ^{[3
]}

Morozov, Alexander N. ^{[4
]}

Yakubovich, Liubov M. ^{[5
]}

Belov, Alexey A. ^{[1
]}

Panfilov, Victor I. ^{[1
]}

Yarovaya, Oksana V. ^{[6
]}

Il'in, Michail M. ^{[7
]}

Sorokin, Vladimir V. ^{[8
]}

Skladnev, Dmitry A. ^{[9
]}

机构：

[1] DI Mendeleyev Univ Chem Technol Russia, Fac Biotechnol & Ind Ecol, Dept Biotechnol, 9 Miusskaya Sq, Moscow 125047, Russia

[2] Mendeleyev Univ Chem Technol Russia, Fac Comp Aided Proc Engn, Dept Cybernet, 9 Miusskaya Sq, Moscow, Russia

[3] Russian Acad Sci, Skryabin Inst Biochem & Physiol Microorganisms, Lab Cytol Microorganisms, 5 Pr Nauki, Pushchino, Moscow Region, Russia

[4] Mendeleyev Univ Chem Technol Russia, Fac Technol Inorgan Subst & High Temp Mat, Dept Technol Inorgan Subst & Electrochem Proc, 9 Miusskaya Sq, Moscow, Russia

[5] Sechenov First Moscow State Med Univ, Dept Analyt Phys & Colloid Chem, Fac Pharm, 8 Bldg 2 Malaya Trubetskaya, Moscow, Russia

[6] Mendeleyev Univ Chem Technol Russia, Dept Colloid Chem, Fac Nat Sci, 9 Miusskaya Sq, Moscow, Russia

[7] Russian Acad Sci, Lab Stereochem Sorpt Proc, Nesmeyanov Inst Organoelement Cpds, 28 Vavilova St, Moscow, Russia

[8] Russian Acad Sci, Biotechnol Res Ctr, Winogradsky Inst Microbiol, CCU Collect Microorganisms UNIQEM, 33 Blvd,2 Leninsky Ave, Moscow, Russia

[9] Russian Acad Sci, Lab Viabil Microorganisms, Winogradsky Inst Microbiol, Biotechnol Res Ctr, 33 Blvd,2 Leninsky Ave, Moscow, Russia

来源：

EXTREMOPHILES | 2018年 / 22卷 / 03期

基金：

俄罗斯科学基金会;

关键词：

Halobacterium salinarum; Spray drying; Carotenoids; Halophiles; artificial neural network; LACTOCOCCUS-LACTIS; MICROORGANISMS; CULTIVATION; RADIATION; SURVIVAL; BACTERIA; STRESSES; CULTURES; STRAIN;

D O I：

10.1007/s00792-018-1013-z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spray drying is appropriate for the preservation of halophilic microorganisms due to the nature of these microorganisms, as they survive in adverse environmental conditions by being encapsulated in salt crystals. Artificial neural networks were in this study used to optimize practically significant spray-drying regimes of the C-50-carotenoids producer Halobacterium salinarum. Immediately after drying, the samples contained up to 54% halobacterial biomass and less than 5% moisture, and the level of preservation of carotenoids was 95-97%. The storage of biomass at 4 A degrees C resulted in the gradual degradation of the carotenoids, which reached 58-64% in the best samples after 1 year. A comprehensive study of changes in halobacteria biomass after spray drying and the nature of the damage provided new data on the survival and preservation of cells and biologically active substances in the various spray-drying regimes and at different storage times.

引用

页码：511 / 523

页数：13

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