Dihydrolipoic acid reduces cytochrome b561 proteins

被引：6
|
作者：
Berczi, Alajos [1 ]
Zimanyi, Laszlo [1 ]
Asard, Han [2 ]
机构：
[1] Hungarian Acad Sci, Inst Biophys, Biol Res Ctr, H-6726 Szeged, Hungary
[2] Univ Antwerp, Dept Biol, B-2020 Antwerp, Belgium
来源：
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS | 2013年 / 42卷 / 2-3期
关键词：
Antioxidants; Ascorbate; Cytochrome b561 proteins; Dihydrolipoic acid; SVD analysis; UV-Vis; TRANSMEMBRANE ELECTRON-TRANSFER; ALPHA-LIPOIC ACID; ESCHERICHIA-COLI; ASCORBIC-ACID; REDOX STATUS; MEMBRANE; B(561); CHROMAFFIN; ANTIOXIDANT; EXPRESSION;
D O I：
10.1007/s00249-012-0812-x
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Cytochrome b561 (Cyt-b561) proteins constitute a family of trans-membrane proteins that are present in a wide variety of organisms. Two of their characteristic properties are the reducibility by ascorbate (ASC) and the presence of two distinct b-type hemes localized on two opposite sides of the membrane. Here we show that the tonoplast-localized and the putative tumor suppressor Cyt-b561 proteins can be reduced by other reductants than ASC and dithionite. A detailed spectral analysis of the ASC-dependent and dihydrolipoic acid (DHLA)-dependent reduction of these two Cyt-b561 proteins is also presented. Our results are discussed in relation to the known antioxidant capability of DHLA as well as its role in the regeneration of other antioxidant compounds of cells. These results allow us to speculate on new biological functions for the trans-membrane Cyt-b561 proteins.
引用
收藏
页码：159 / 168
页数：10
相关论文
共 50 条
  • [21] Heterologous expression and site-directed mutagenesis of an ascorbate-reducible cytochrome b561
    Bérczi, A
    Su, D
    Lakshminarasimhan, M
    Vargas, A
    Asard, H
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2005, 443 (1-2) : 82 - 92
  • [22] Identification of an ascorbate-dependent cytochrome b of the tonoplast membrane sharing biochemical features with members of the cytochrome b561 family
    Valeria Preger
    Sandra Scagliarini
    Paolo Pupillo
    Paolo Trost
    Planta, 2005, 220 : 365 - 375
  • [23] Cytochrome b561 is not fatty acylated but acetylated at amino terminus in chromaffin vesicle membranes:: an approach for the identification of posttranslational modification of transmembrane proteins
    Nakamura, M
    Takeuchi, F
    Tsubaki, M
    PROTOPLASMA, 2003, 221 (1-2) : 41 - 46
  • [24] Tonoplast cytochrome b561 is a transmembrane ascorbate-dependent monodehydroascorbate reductase: functional characterization of electron currents in plant vacuoles
    Gradogna, Antonella
    Lagostena, Laura
    Beltrami, Sara
    Tosato, Edoardo
    Picco, Cristiana
    Scholz-Starke, Joachim
    Sparla, Francesca
    Trost, Paolo
    Carpaneto, Armando
    NEW PHYTOLOGIST, 2023, 238 (05) : 1957 - 1971
  • [25] Roles of conserved Arg72 and Tyr71 in the ascorbate-specific transmembrane electron transfer catalyzed by Zea mays cytochrome b561
    Rahman, Motiur Md.
    Nakanishi, Nobuyuki
    Sakamoto, Yoichi
    Hori, Hiroshi
    Hase, Toshiharu
    Park, Sam-Yong
    Tsubaki, Motonari
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2013, 115 (05) : 497 - 506
  • [26] Reversely-oriented cytochrome b561 in reconstituted vesicles catalyzes transmembrane electron transfer and supports extravesicular dopamine β-hydroxylase activity
    Seike, Y
    Takeuchi, F
    Tsubaki, M
    JOURNAL OF BIOCHEMISTRY, 2003, 134 (06): : 859 - 867
  • [27] Electron Transfer Reactions of Candidate Tumor Suppressor 101F6 Protein, a Cytochrome b561 Homologue, with Ascorbate and Monodehydroascorbate Radical
    Recuenco, Mariam C.
    Rahman, Md. Motiur
    Takeuchi, Fusako
    Kobayashi, Kazuo
    Tsubaki, Motonari
    BIOCHEMISTRY, 2013, 52 (21) : 3660 - 3668
  • [28] Human Heart Rate Heritability of Resting and Stress Values in Twin Pairs, and Influence of Genetic Variation in the Adrenergic Pathway at a Microribonucleic Acid MicroRNA Motif in the 3′-UTR of Cytochrome b561
    Zhang, Kuixing
    Deacon, Dekker C.
    Rao, Fangwen
    Schork, Andrew J.
    Fung, Maple M.
    Waalen, Jill
    Schork, Nicholas J.
    Nievergelt, Caroline M.
    Chi, Neil C.
    O'Connor, Daniel T.
    JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2014, 63 (04) : 358 - 368
  • [29] Direct Recording of Trans-Plasma Membrane Electron Currents Mediated by a Member of the Cytochrome b561 Family of Soybean
    Picco, Cristiana
    Scholz-Starke, Joachim
    Festa, Margherita
    Costa, Alex
    Sparla, Francesca
    Trost, Paolo
    Carpaneto, Armando
    PLANT PHYSIOLOGY, 2015, 169 (02) : 986 - 995
  • [30] Three mammalian cytochromes b561 are ascorbate-dependent ferrireductases
    Su, Dan
    Asard, Han
    FEBS JOURNAL, 2006, 273 (16) : 3722 - 3734
12345