Application of recombinant antibody fragments for troponin I measurements

被引：2
作者：
Altshuler, E. P. [1 ]
Vylegzhanina, A. V. [2 ]
Katrukha, I. A. [1 ]
Bereznikova, A. V. [1 ]
Serebryanaya, D. V. [1 ]
机构：
[1] Moscow MV Lomonosov State Univ, Fac Biol, Moscow 119991, Russia
[2] Bialexa Ltd, Moscow 117149, Russia
来源：
BIOCHEMISTRY-MOSCOW | 2012年 / 77卷 / 12期
关键词：
monoclonal antibodies; recombinant antibodies; troponin I; affinity; fluorescence immunoassay; biotinylation; ACUTE MYOCARDIAL-INFARCTION; CARDIAC TROPONIN; INTERFERENCE; DIGESTION; ASSAY;
D O I：
10.1134/S0006297912120036
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The cardiac isoform of troponin I is a reliable biomarker of damaged cardiomyocytes that accompanies such severe cardiovascular diseases as myocardial infarction. Monoclonal antibody 19C7 recognizes troponin I in the blood-stream with high affinity and specificity. Recombinant antibodies can be used to improve detection systems based on monoclonal antibodies produced with hybridoma technology. In the present study, we compare the properties of monoclonal anti-body 19C7 and its recombinant fragments. It is shown that the recombinant antibody fragments demonstrate similar affinity values as monoclonal antibodies and can be applied for troponin I detection.
引用
收藏
页码：1362 / 1367
页数：6
相关论文
共 15 条
[1]   Papain digestion of different mouse IgG subclasses as studied by electrospray mass spectrometry [J].
Adamczyk, M ;
Gebler, JC ;
Wu, J .
JOURNAL OF IMMUNOLOGICAL METHODS, 2000, 237 (1-2) :95-104
[2]   Myocardial infarction redefined -: A consensus Document of the Joint European Society of Cardiology/American College of Cardiology Committee for the Redefinition of Myocardial Infarction [J].
Alpert, JS ;
Antman, E ;
Apple, F ;
Armstrong, PW ;
Bassand, JP ;
de Luna, AB ;
Beller, G ;
Breithardt, G ;
Chaitman, BR ;
Clemmensen, P ;
Falk, E ;
Fishbein, MC ;
Galvani, M ;
Garson, A ;
Grines, C ;
Hamm, C ;
Jaffe, A ;
Katus, H ;
Kjekshus, J ;
Klein, W ;
Klootwijk, P ;
Lenfant, C ;
Levy, D ;
Levy, RI ;
Luepker, R ;
Marcus, F ;
Näslund, U ;
Ohman, M ;
Pahlm, O ;
Poole-Wilson, P ;
Popp, R ;
Alto, P ;
Pyörälä, K ;
Ravkilde, J ;
Rehnquist, N ;
Roberts, W ;
Roberts, R ;
Roelandt, J ;
Rydén, L ;
Sans, S ;
Simoons, ML ;
Thygesen, K ;
Tunstall-Pedoe, H ;
Underwood, R ;
Uretsky, BF ;
Van de Werf, F ;
Voipio-Pulkki, LM ;
Wagner, G ;
Wallentin, L ;
Wijns, W .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2000, 36 (03) :959-969
[3]   Generation of recombinant antibodies and means for increasing their affinity [J].
Altshuler, E. P. ;
Serebryanaya, D. V. ;
Katrukha, A. G. .
BIOCHEMISTRY-MOSCOW, 2010, 75 (13) :1584-1605
[4]   Use of high-capacity surface with oriented recombinant antibody fragments in a 5-min immunoassay for thyroid-stimulating hormone [J].
Brockmann, Eeva-Christine ;
Vehniainen, Markus ;
Pettersson, Kim .
ANALYTICAL BIOCHEMISTRY, 2010, 396 (02) :242-249
[5]   CARDIAC-SPECIFIC TROPONIN-I RADIOIMMUNOASSAY IN THE DIAGNOSIS OF ACUTE MYOCARDIAL-INFARCTION [J].
CUMMINS, B ;
AUCKLAND, ML ;
CUMMINS, P .
AMERICAN HEART JOURNAL, 1987, 113 (06) :1333-1344
[6]   Myocardial infarction redefined: the new ACC/ESC definition, based on cardiac troponin, increases the apparent incidence of infarction [J].
Ferguson, JL ;
Beckett, GJ ;
Stoddart, M ;
Walker, SW ;
Fox, KAA .
HEART, 2002, 88 (04) :343-347
[7]  
Filatov VL, 1998, BIOCHEM MOL BIOL INT, V45, P1179
[8]   An immunoprecipitation coupled with fluorescent Western blot analysis for the characterization of a model secondary serum cardiac troponin I reference material [J].
He, Hua-Jun ;
Lowenthal, Mark S. ;
Cole, Kenneth D. ;
Bunk, David ;
Wang, Lili .
CLINICA CHIMICA ACTA, 2011, 412 (1-2) :107-111
[9]   Early markers of myocardial injury: cTnI is enough [J].
Ilva, Tuomo ;
Lund, Juha ;
Porela, Pekka ;
Mustonen, Harri ;
Voipio-Pulkki, Liisa-Maria ;
Eriksson, Susann ;
Pettersson, Kim ;
Tanner, Pirjo ;
Pulkki, Kari .
CLINICA CHIMICA ACTA, 2009, 400 (1-2) :82-85
[10]  
Katrukha AG, 1997, CLIN CHEM, V43, P1379
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