Variation in the AvrSr35 gene determines Sr35 resistance against wheat stem rust race Ug99

被引：161

作者：

Salcedo, Andres ^{[1
]}

Rutter, William ^{[1
,9
]}

Wang, Shichen ^{[1
,10
]}

Akhunova, Alina ^{[2
]}

Bolus, Stephen ^{[3
]}

Chao, Shiaoman ^{[4
]}

Anderson, Nickolas ^{[1
]}

De Soto, Monica Fernandez ^{[2
]}

Rouse, Matthew ^{[5
,6
]}

Szabo, Les ^{[5
,6
]}

Bowden, Robert L. ^{[7
]}

Dubcovsky, Jorge ^{[3
,8
]}

Akhunov, Eduard ^{[1
]}

机构：

[1] Kansas State Univ, Dept Plant Pathol, Throckmorton Hall, Manhattan, KS 66506 USA

[2] Kansas State Univ, Integrated Genom Facil, Manhattan, KS 66506 USA

[3] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA

[4] USDA ARS, Cereal Crops Res Unit, Fargo, ND 58102 USA

[5] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA

[6] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA

[7] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA

[8] HHMI, Chevy Chase, MD 20815 USA

[9] USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA

[10] Texas A&M Univ, Texas A&M Engn Expt Stn, AgriLife Ctr Bioinformat & Genom Syst Engn, College Stn, TX 77845 USA

来源：

SCIENCE | 2017年 / 358卷 / 6370期

基金：

美国食品与农业研究所;

关键词：

D O I：

10.1126/science.aao7294

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Puccinia graminis f. sp. tritici (Pgt) causes wheat stem rust, a devastating fungal disease. The Sr35 resistance gene confers immunity against this pathogen's most virulent races, including Ug99. We used comparative whole-genome sequencing of chemically mutagenized and natural Pgt isolates to identify a fungal gene named AvrSr35 that is required for Sr35 avirulence. The AvrSr35 gene encodes a secreted protein capable of interacting with Sr35 and triggering the immune response. We show that the origin of Pgt isolates virulent on Sr35 is associated with the nonfunctionalization of the AvrSr35 gene by the insertion of a mobile element. The discovery of AvrSr35 provides a new tool for Pgt surveillance, identification of host susceptibility targets, and characterization of the molecular determinants of immunity in wheat.

引用

页码：1604 / 1606

页数：3

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