Characterization of 11 tetranucleotide microsatellite loci for the little brown bat (Myotis lucifugus) based on in silico genome sequences

被引：6
作者：
Burns, Lynne E. [1 ]
Broders, Hugh G. [2 ]
Frasier, Timothy R. [2 ]
机构：
[1] Dalhousie Univ, Dept Biol, Life Sci Ctr, Halifax, NS B3H 4J1, Canada
[2] St Marys Univ, Dept Biol, Halifax, NS B3H 3C3, Canada
来源：
CONSERVATION GENETICS RESOURCES | 2012年 / 4卷 / 03期
关键词：
Microsatellites; Population structure; Myotis lucifugus; In silico;
D O I：
10.1007/s12686-012-9615-8
中图分类号：
X176 [生物多样性保护];
学科分类号：
090705 ;
摘要：
Using an in silico approach, we identified tetranucleotide microsatellite loci from an existing whole genome shotgun DNA sequence of the little brown bat (Myotis lucifugus). Eleven loci were polymorphic, and exhibited a range from 4 to 25 alleles per locus (mean = 11). Observed heterozygosities ranged from 46 to 94%. Primers for the 11 loci were also tested for cross-species amplification in Myotis septentrionalis. These microsatellites will be useful for genetics-based studies of M. lucifugus, which are of increasing importance due to their conservation concern regarding the spread of white-nose syndrome.
引用
收藏
页码：653 / 655
页数：3
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