Continuous throughput and long-term observation of single-molecule FRET without immobilization

被引：45

作者：

Tyagi, Swati ^{[1
]}

VanDelinder, Virginia ^{[1
]}

Banterle, Niccolo ^{[1
]}

Fuertes, Gustavo ^{[1
]}

Milles, Sigrid ^{[1
]}

Agez, Morgane ^{[1
]}

Lemke, Edward A. ^{[1
]}

机构：

[1] EMBL, Struct & Computat Biol Unit, Heidelberg, Germany

来源：

NATURE METHODS | 2014年 / 11卷 / 03期

关键词：

CONFORMATIONAL DYNAMICS; FLUORESCENCE; TRANSGLUTAMINASE; SYSTEM;

D O I：

10.1038/nmeth.2809

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present an automated microfluidic platform that performs multisecond observation of single molecules with millisecond time resolution while bypassing the need for immobilization procedures. With this system, we confine biomolecules to a thin excitation field by reversibly collapsing microchannels to nanochannels. We demonstrate the power of our method by studying a variety of complex nucleic acid and protein systems, including DNA Holliday junctions, nucleosomes and human transglutaminase 2.

引用

页码：297 / U358

页数：7

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