Muscovite (mica) allows the characterisation of supported Bilayers by ellipsometry and confocal fluorescence correlation spectroscopy

被引：27

作者：

Benes, M

Billy, D

Hermens, WT

Hof, M

机构：

[1] Acad Sci Czech Republ, J Heyrovsky Inst Phys Chem, Ctr Complex Mol Syst & Biomol, CZ-18223 Prague, Czech Republic

[2] Medtron Bakken Res Ctr, NL-6229 GW Maastricht, Netherlands

[3] Univ Maastricht, Cardiovasc Res Inst Maastricht, NL-6200 MD Maastricht, Netherlands

来源：

BIOLOGICAL CHEMISTRY | 2002年 / 383卷 / 02期

关键词：

bodipy; borosillcate glass; lateral diffusion; microscopy; oregon green; phospholipid vesicles;

D O I：

10.1515/BC.2002.037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate for the first time that ellipsometry and confocal fluorescence correlation spectroscopy (FGS) are complementary methods for the characterisation of supported planar phospholipid bilayers (SPBs) formed on mica, a mineral used in atomic force microscopy investigations of SPBs. Addition of small unilamellar vesicles containing 20% dioleoylphosphatidylserine (DOPS) and 80% dioleoyl-phosphatidylcholine (DOPC) to an oxidised borosilicate surface, on the other hand, results in a planar lipid system characterised by lateral diffusion coefficients which are three time smaller than those obtained for SPBs. Moreover, seven labelled phospholipids were tested for their suitability in the FCS characterisation of vesicles as well as of SPBs.

引用

页码：337 / 341

页数：5

共 32 条

[1] TOTAL INTERNAL-REFLECTION FLUORESCENT MICROSCOPY [J].

AXELROD, D ;

THOMPSON, NL ;

BURGHARDT, TP .

JOURNAL OF MICROSCOPY-OXFORD, 1983, 129 (JAN) :19-28

[2] ALLOGENEIC STIMULATION OF CYTO-TOXIC T-CELLS BY SUPPORTED PLANAR MEMBRANES [J].

BRIAN, AA ;

MCCONNELL, HM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (19) :6159-6163

[3] Interaction of phospholipid vesicles with smooth metal-oxide surfaces [J].

Csúcs, G ;

Ramsden, JJ .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1998, 1369 (01) :61-70

[4] TEMPERATURE-DEPENDENT TRANSITIONS IN SEVERAL PHOSPHOLIPIDS MEASURED BY ELLIPSOMETRY [J].

CUYPERS, PA ;

JANSSEN, MP ;

KOP, JMM ;

HERMENS, WT ;

HEMKER, HC .

SURFACE SCIENCE, 1980, 96 (1-3) :555-563

[5] ROTATIONAL BROWNIAN-MOTION AND FLUORESCENCE INTENSITY FLUCTUATIONS [J].

EHRENBER.M ;

RIGLER, R .

CHEMICAL PHYSICS, 1974, 4 (03) :390-401

[6] FLUORESCENCE CORRELATION SPECTROSCOPY .1. CONCEPTUAL BASIS AND THEORY [J].

ELSON, EL ;

MAGDE, D .

BIOPOLYMERS, 1974, 13 (01) :1-27

[7] LATERAL DIFFUSION IN PLANAR LIPID BILAYERS [J].

FAHEY, PF ;

KOPPEL, DE ;

BARAK, LS ;

WOLF, DE ;

ELSON, EL ;

WEBB, WW .

SCIENCE, 1977, 195 (4275) :305-306

[8] PRODUCTION OF THROMBIN AS A PROBE FOR MIXING OF PHOSPHOLIPIDS IN MEMBRANES ON SOLID SUPPORTS [J].

GIESEN, PLA ;

HEMKER, HC ;

HERMENS, WT .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1995, 1237 (01) :43-48

[9]

HUANG DT, 1994, CHEM BIOL, V1, P67

[10] EFFECT OF BOVINE PROTHROMBIN FRAGMENT-1 ON THE TRANSLATIONAL DIFFUSION OF PHOSPHOLIPIDS IN LANGMUIR-BLODGETT MONOLAYERS [J].

HUANG, ZP ;

PEARCE, KH ;

THOMPSON, NL .

BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1112 (02) :259-265

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