Observation of salt effects on hydration water of lysozyme in aqueous solution using terahertz time-domain spectroscopy

被引:21
作者
Aoki, Katsuyoshi [1 ]
Shiraki, Kentaro [2 ]
Hattori, Toshiaki [1 ]
机构
[1] Univ Tsukuba, Inst Appl Phys, Tsukuba, Ibaraki 3058573, Japan
[2] Univ Tsukuba, Fac Pure & Appl Sci, Tsukuba, Ibaraki 3058573, Japan
关键词
ABSORPTION-SPECTROSCOPY; HOFMEISTER SERIES; ION HYDRATION; LIQUID WATER; PROTEIN; DYNAMICS; STABILITY; SPECTRA;
D O I
10.1063/1.4826699
中图分类号
O59 [应用物理学];
学科分类号
摘要
Terahertz time-domain spectroscopy was used to investigate the salt effect of ammonium sulfate on the dynamics of hydration water of lysozyme in aqueous solution. The absorption coefficient of lysozyme aqueous solutions containing salt was subtracted by that of the water and ammonium sulfate contained in the lysozyme solution. The results revealed that ammonium sulfate increases the absorption coefficient of the hydration water, which indicates that the dynamics of the hydration water becomes faster and/or the number of hydration water molecules decreases with increasing ammonium sulfate concentration. (C) 2013 AIP Publishing LLC.
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页数:4
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