Glucose biosensor based on disposable electrochemical paper-based transducers fully fabricated by screen-printing

被引:78
作者
Lamas-Ardisana, P. J. [1 ]
Martinez-Paredes, G. [1 ]
Anorga, L. [1 ]
Grande, H. J. [1 ]
机构
[1] CIDETEC, Parque Tecnol San Sebastian,P Miramon 196, Donostia San Sebastian 20014, Spain
关键词
Paper-based device; Screen-printing; Fabrication; Electrode; Glucose biosensor; Soft-drinks; DEVICES; ELECTRODES;
D O I
10.1016/j.bios.2018.02.061
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
This paper describes a new approach for the massive production of electrochemical paper-based analytical devices (ePADs). These devices are fully fabricated by screen-printing technology and consist of a lineal microfluidic channel delimited by hydrophobic walls (patterned with diluted ultraviolet screen-printing ink in chromatographic paper grade 4) and a three-electrode system (printed with carbon and/or Ag/AgCl conductive inks). The printing process was characterised and optimized for pattern each layer with only one squeeze sweep. These ePADs were used as transducers to develop a glucose biosensor. Ionic strength/pH buffering salts, electrochemical mediator (ferricyanide) and enzyme (glucose dehydrogenase FAD-dependent) were separately stored along the microfluidic channel in order to be successively dissolved and mixed after the sample dropping at the entrance. The analyses required only 10 mu l and the biosensors showed good reproducibility (RSD = 6.2%, n = 10) and sensitivity (0.426 C/M cm(2)), wide linear range (0.5-50 mM; r(2) = 0.999) and low limit of detection (0.33 mM). Furthermore, the new biosensor was applied for glucose determination in five commercial soft-drinks without any sample treatment before the analysis. These samples were also analysed with a commercial enzymatic-kit assay. The results indicated that both methods provide accurate results.
引用
收藏
页码:8 / 12
页数:5
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