Transcriptional activation by Arabidopsis GT-1 may be through interaction with TFIIA-TBP-TATA complex

被引：100
作者：
Le Gourrierec, J [1 ]
Li, YF [1 ]
Zhou, DX [1 ]
机构：
[1] Univ Picardie, Lab Genet Mol Plantes, F-80039 Amiens, France
来源：
PLANT JOURNAL | 1999年 / 18卷 / 06期
关键词：
D O I：
10.1046/j.1365-313x.1999.00482.x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
GT-1 belongs to the class of trihelix DNA-binding proteins and binds to a promoter sequence found in many different genes. Data presented in this report show that GT-1 contains a trans-activation function in yeast and in plant cells. However, in tobacco BY-2 protoplasts, this activity functions only when an internal region containing the DNA-binding domain is deleted. Gel-shift and co-immunoprecipitation assays have revealed that GT-1 can interact with and stabilize the TFIIA-TBP-TATA complex. These results suggest that GT-1 may activate transcription through direct interaction with the transcriptional pre-initiation complex.
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页码：663 / 668
页数：6
相关论文
共 23 条
[1]   The PR-1a promoter contains a number of elements that bind GT-1-like nuclear factors with different affinity [J].
Buchel, AS ;
Molenkamp, R ;
Bol, JF ;
Linthorst, HJM .
PLANT MOLECULAR BIOLOGY, 1996, 30 (03) :493-504
[2]  
Cardoso MIL, 1997, MOL GEN GENET, V256, P674
[3]   Assembly of the isomerized TFIIA-TFIID-TATA ternary complex is necessary and sufficient for gene activation [J].
Chi, TH ;
Carey, M .
GENES & DEVELOPMENT, 1996, 10 (20) :2540-2550
[4]  
Clemens KE, 1996, MOL CELL BIOL, V16, P4656
[5]   POLLEN SPECIFICITY ELEMENTS RESIDE IN 30 BP OF THE PROXIMAL PROMOTERS OF 2 POLLEN-EXPRESSED GENES [J].
EYAL, Y ;
CURIE, C ;
MCCORMICK, S .
PLANT CELL, 1995, 7 (03) :373-384
[6]   CHARACTERIZATION OF A GENE ENCODING A DNA-BINDING PROTEIN WITH SPECIFICITY FOR A LIGHT-RESPONSIVE ELEMENT [J].
GILMARTIN, PM ;
MEMELINK, J ;
HIRATSUKA, K ;
KAY, SA ;
CHUA, NH .
PLANT CELL, 1992, 4 (07) :839-849
[7]   SEQUENCE-SPECIFIC INTERACTIONS OF A PEA NUCLEAR FACTOR WITH LIGHT-RESPONSIVE ELEMENTS UPSTREAM OF THE RBCS-3A GENE [J].
GREEN, PJ ;
KAY, SA ;
CHUA, NH .
EMBO JOURNAL, 1987, 6 (09) :2543-2549
[8]   MOLECULAR DISSECTION OF GT-1 FROM ARABIDOPSIS [J].
HIRATSUKA, K ;
WU, XD ;
FUKUZAWA, H ;
CHUA, NH .
PLANT CELL, 1994, 6 (12) :1805-1813
[9]  
JEFFERSON RA, 1987, EMBO J, V6, P3901
[10]   DA-complex assembly activity required for VP16C transcriptional activation [J].
Kobayashi, N ;
Horn, PJ ;
Sullivan, SM ;
Triezenberg, SJ ;
Boyer, TG ;
Berk, AJ .
MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (07) :4023-4031
123