Peroxidase catalyzed formation of azine pigments - a convenient and sensitive method for the identification of human cells with positive myeloperoxidase reactivity

被引：19

作者：

Bodtke, A

Pfeiffer, WD

Ahrens, N

Langer, P

机构：

[1] Univ Greifswald, Inst Chem & Biochem, D-17487 Greifswald, Germany

[2] Univ Greifswald, Neurol Klin, D-17487 Greifswald, Germany

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2004年 / 14卷 / 06期

关键词：

enzyme; peroxidase; azine; pigment; cell;

D O I：

10.1016/j.bmcl.2004.01.001

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A new and efficient method for the identification of human cells with positive myeloperoxidase reactivity was developed. This method is based on the myeloperoxidase catalyzed formation of p-naphthoquinone-thiazol-2-on-azine pigments and offers a number of advantages compared to standard methods. In addition, we report the horseradish peroxidase (HRP) catalyzed oxidative coupling of 2-hydrazono-4-thiazolines with alpha-naphthol. This transformation allows a new and efficient synthesis of p-naphthoquinone-thiazol-2-on-azines under mild conditions. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：1509 / 1511

页数：3

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