Immobilization of Bacterial Luciferase into Poly(N-isopropylacrylamide) Film for Electrochemical Control of a Bioluminescence Reaction

被引：4

作者：

Kawanami, Yoji ^{[1
]}

Yamasaki, Shinya ^{[2
]}

Yamada, Shuto ^{[3
]}

Takehara, Ko ^{[1
]}

机构：

[1] Kyushu Univ, Fac Sci, Dept Chem, Fukuoka 8128581, Japan

[2] Japan Atom Energy Agcy, Adv Sci Res Ctr, Tokai, Ibaraki 3191195, Japan

[3] Kyushu Univ, Fac Arts & Sci, Fukuoka 8190395, Japan

来源：

ANALYTICAL SCIENCES | 2012年 / 28卷 / 10期

关键词：

FIREFLY LUCIFERASE; ANESTHETICS; BINDING;

D O I：

10.2116/analsci.28.1013

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A poly(N-isopropylacrylamide) film was modified on an indium-tin oxide electrode in order to immobilize bacterial luciferase (BL) on the electrode surface. By using the modified electrode, flavin mononucleotide (FMN) was electrochemically reduced to FMNH2, which is one of the substrates of the BL luminescence reaction, to control the bioluminescence reaction by BL. The BL reaction in the modified film could be promoted and controlled by the electrochemical generation of FMNH2. This BL luminescence system was evaluated as a model system for the inhibitory assay of hydrophobic molecules on protein functions.

引用

页码：1013 / 1015

页数：3

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