Irradiation by a Combination of Different Peak-Wavelength Ultraviolet-Light Emitting Diodes Enhances the Inactivation of Influenza A Viruses

被引：19

作者：

Kojima, Mizuki ^{[1
]}

Mawatari, Kazuaki ^{[1
]}

Emoto, Takahiro ^{[2
]}

Nishisaka-Nonaka, Risa ^{[1
]}

Thi Kim Ngan Bui ^{[1
]}

Shimohata, Takaaki ^{[1
]}

Uebanso, Takashi ^{[1
]}

Akutagawa, Masatake ^{[2
]}

Kinouchi, Yohsuke ^{[2
]}

Wada, Takahiro ^{[3
]}

Okamoto, Masayuki ^{[3
]}

Ito, Hiroshi ^{[3
]}

Tojo, Kenji ^{[3
]}

Daidoji, Tomo ^{[4
]}

Nakaya, Takaaki ^{[4
]}

Takahashi, Akira ^{[1
]}

机构：

[1] Tokushima Univ, Inst Biomed Sci, Dept Prevent Environm & Nutr, Grad Sch, Kuramoto Cho 3-18-15, Tokushima, Tokushima 7708503, Japan

[2] Grad Sch Technol Ind & Social Sci, Minamijyousanjima Cho 2-1, Tokushima, Tokushima 7708506, Japan

[3] Nihon Funen Co Ltd, 179-1 Mitsujima Shinden,Kawashima Cho, Yoshinogawa City, Tokushima 7793394, Japan

[4] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Dept Infect Dis, Kamigyo Ku, 465 Kajii Cho, Kyoto 6028566, Japan

来源：

MICROORGANISMS | 2020年 / 8卷 / 07期

关键词：

light emitting diode; ultraviolet; influenza A virus; DISINFECTION PERFORMANCE; DRINKING-WATER; UVC-LEDS; RNA; HEMAGGLUTININ; VACCINE; REPAIR; H5N1; MS2; DNA;

D O I：

10.3390/microorganisms8071014

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Influenza A viruses (IAVs) pose a serious global threat to humans and their livestock. This study aimed to determine the ideal irradiation by ultraviolet-light emitting diodes (UV-LEDs) for IAV disinfection. We irradiated the IAV H1N1 subtype with 4.8 mJ/cm(2)UV using eight UV-LEDs [peak wavelengths (WL) = 365, 310, 300, 290, 280, 270, and 260 nm)] or a mercury low pressure (LP)-UV lamp (Peak WL = 254 nm). Inactivation was evaluated by the infection ratio of Madin-Darby canine kidney (MDCK) cells or chicken embryonated eggs. Irradiation by the 260 nm UV-LED showed the highest inactivation among all treatments. Because the irradiation-induced inactivation effects strongly correlated with damage to viral RNA, we calculated the correlation coefficient (R-AE) between the irradiant spectrum and absorption of viral RNA. TheR(AE)scores strongly correlated with the inactivation by the UV-LEDs and LP-UV lamp. To increase theR(AE)score, we combined three different peak WL UV-LEDs (hybrid UV-LED). The hybrid UV-LED (R-AE= 86.3) significantly inactivated both H1N1 and H6N2 subtypes to a greater extent than 260 nm (R-AE= 68.6) or 270 nm (R-AE= 42.2) UV-LEDs. TheR(AE)score is an important factor for increasing the virucidal effects of UV-LED irradiation.

引用

页码：1 / 15

页数：15

共 42 条

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