Changes in barrier health status of the gill for grass carp (Ctenopharyngodon idella) during valine deficiency: Regulation of tight junction protein transcript, antioxidant status and apoptosis-related gene expression

被引:47
作者
Feng, Lin [1 ,2 ,3 ]
Luo, Jian-Bo [1 ]
Jiang, Wei-Dan [1 ,2 ,3 ]
Liu, Yang [1 ,2 ,3 ]
Wu, Pei [1 ,2 ,3 ]
Jiang, Jun [1 ,2 ,3 ]
Kuang, Sheng-Yao [4 ]
Tang, Ling [4 ]
Zhang, Yong-An [5 ]
Zhou, Xiao-Qiu [1 ,2 ,3 ]
机构
[1] Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Peoples R China
[2] Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Peoples R China
[3] Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Peoples R China
[4] Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China
[5] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词
Valine deficiency; Tight junction protein; Antioxidant defense; Signaling molecule; Apoptosis; NF-KAPPA-B; MUCOSAL IMMUNE-SYSTEM; SALMON SALMO-SALAR; FATTY-ACID-COMPOSITION; CHAIN AMINO-ACIDS; OXIDATIVE STRESS; CYPRINUS-CARPIO; FRESH-WATER; FISH; CELLS;
D O I
10.1016/j.fsi.2015.04.023
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
This study investigated the effects of dietary valine on tight junction protein transcription, antioxidant status and apoptosis on grass carp gills (Ctenopharyngodon idella). Fish were fed six different experimental diets containing graded levels of valine (4.3, 8.0, 10.6, 13.1, 16.7, 19.1 g/kg). The results indicated that valine deficiency decreased Claudin b, Claudin 3, Occludin and ZO-1 transcription and increased Claudin 15 expression in the fish gill (P < 0.05). These effects were partly due to the down-regulation of interleukin 10 (IL-10), transforming growth factor 131 (TGF-beta 1) and I kappa B alpha and the up-regulation of relative mRNA expression of interleukin 1 beta (IL-1 beta), interleukin 8 (IL-8), tumor necrosis factor-alpha (TNF-alpha) and nuclear factor kappa B P65 (NF-kappa B P65) (P < 0.05). However, valine deficiency and valine supplementation did not have a significant effect on Claudin c and Claudin 12 expression in grass carp gills (P > 0.05). Valine deficiency also disrupted antioxidant status in the gill by decreasing anti-superoxide radicals and hydroxyl radical capacity, glutathione contents and the activities and mRNA levels of Cu/Zn superoxide dismutase (SOD1), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST) (P < 0.05). These results may be ascribed to the down-regulation of NF-E2-related factor 2 (Nrf2), target of rapamycin (TOR) and ribosomal protein S6 kinase 1 (S6K1) and the up-regulation of Kelch-like-ECH-associated protein 1 (Keap1) (P < 0.05). Additionally, valine deficiency induced DNA fragmentation via the up-regulation of Caspase 3, Caspase 8 and Caspase 9 expressions (P < 0.05). These results may be ascribed to the improvement in ROS levels in the fish gill (P < 0.05). Taken together, the results showed that valine deficiency impaired the structural integrity of fish gill by disrupted fish antioxidant defenses and regulating the expression of tight junction protein, cytokines, antioxidant enzymes, NF-kappa B p65, I kappa B alpha, TOR, Nrf2, Keap1 and apoptosis-related genes in the fish gill. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码:239 / 249
页数:11
相关论文
共 89 条
[1]  
AEBI H, 1984, METHOD ENZYMOL, V105, P121
[2]   IL-1β causes an increase in intestinal epithelial tight junction permeability [J].
Al-Sadi, Rana M. ;
Ma, Thomas Y. .
JOURNAL OF IMMUNOLOGY, 2007, 178 (07) :4641-4649
[3]   Analysis of protein carbonyls in meat products by using the DNPH-method, fluorescence spectroscopy and liquid chromatography-electrospray ionisation-mass spectrometry (LC-ESI-MS) [J].
Armenteros, M. ;
Heinonen, M. ;
Illilainen, V. ;
Toldra, F. ;
Estevez, M. .
MEAT SCIENCE, 2009, 83 (01) :104-112
[4]   Sestrins Activate Nrf2 by Promoting p62-Dependent Autophagic Degradation of Keap1 and Prevent Oxidative Liver Damage [J].
Bae, Soo Han ;
Sung, Su Haeng ;
Oh, Sue Young ;
Lim, Jung Mi ;
Lee, Se Kyoung ;
Park, Young Nyun ;
Lee, Hye Eun ;
Kang, Dongmin ;
Rhee, Sue Goo .
CELL METABOLISM, 2013, 17 (01) :73-84
[5]   CONSTITUTIVE NF-KAPPA-B ACTIVATION, ENHANCED GRANULOPOIESIS, AND NEONATAL LETHALITY IN I-KAPPA-B-ALPHA-DEFICIENT MICE [J].
BEG, AA ;
SHA, WC ;
BRONSON, RT ;
BALTIMORE, D .
GENES & DEVELOPMENT, 1995, 9 (22) :2736-2746
[6]   STRUCTURAL STUDIES OF ALPHA-MELANOCYTE-STIMULATING HORMONE AND A NOVEL BETA-MELANOCYTE-STIMULATING HORMONE FROM NEUROINTERMEDIATE LOBE OF PITUITARY OF DOGFISH SQUALUS-ACANTHIAS [J].
BENNETT, HPJ ;
LOWRY, PJ ;
MCMARTIN, C ;
SCOTT, AP .
BIOCHEMICAL JOURNAL, 1974, 141 (02) :439-444
[7]   LIPID AND FATTY-ACID COMPOSITION OF MILKFISH (CHANOS-CHANOS FORSSKAL) GROWN IN FRESH-WATER AND SEAWATER [J].
BORLONGAN, IG ;
BENITEZ, LV .
AQUACULTURE, 1992, 104 (1-2) :79-89
[8]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[9]   Effects of water temperature and dissolved oxygen on daily feed consumption, feed utilization and growth of channel catfish (Ictalurus punctatus) [J].
Buentello, JA ;
Gatlin, DM ;
Neill, WH .
AQUACULTURE, 2000, 182 (3-4) :339-352
[10]   Cytokine regulation of tight junctions [J].
Capaldo, Christopher T. ;
Nusrat, Asma .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2009, 1788 (04) :864-871