The establishment of good experimental models for Theileria sp. infection is important for theileriosis research. Routinely, infection of ticks is accomplished by feeding on parasite-infected animals (sheep, cows and horses), which raises practical and ethical problems, driving the search for alternative methods of tick infection. Artificial tick feeding systems are based mainly on rearing ticks on host-derived or hand-made artificial membranes. We developed a modified feeding assay for infecting nymphal stages of Hyalomma anatolicum ticks with Theileria lestoquardi, a highly pathogenic parasite of sheep. We compared two different membranes: an artificial silicone membrane and a natural alternative using mouse skin. We observed high attachment rates with mouse skin, whereas in vitro feeding of H. anatolicum nymphs on silicone membranes was unsuccessful. We could infect H. anatolicum nymphs with T. lestoquardi and the emerging adult ticks transmitted infective parasites to sheep. In contrast, similar infections with Rhipicephalus bursa, a representative tick with short mouth-parts that was proposed as a vector for T. lestoquardi, appeared not to be a competent vector tick species. This is the first report of an experimentally controlled infection of H. anatolicum with T. lestoquardi and opens avenues to explore tick-parasite dynamics in detail.
机构:
IREC CSIC UCLM JCCM, SaBio, Inst Invest Recursos Cineget, Ciudad Real 13005, SpainInst Higiene & Med Trop, P-1349008 Lisbon, Portugal
Perez de la Lastra, Jose M.
;
Alberdi, Pilar
论文数: 0引用数: 0
h-index: 0
机构:
IREC CSIC UCLM JCCM, SaBio, Inst Invest Recursos Cineget, Ciudad Real 13005, SpainInst Higiene & Med Trop, P-1349008 Lisbon, Portugal
Alberdi, Pilar
;
Domingos, Ana
论文数: 0引用数: 0
h-index: 0
机构:
Inst Higiene & Med Trop, Ctr Malaria & Outras Doencas Tropicais, P-1349008 Lisbon, PortugalInst Higiene & Med Trop, P-1349008 Lisbon, Portugal
Domingos, Ana
;
de la Fuente, Jose
论文数: 0引用数: 0
h-index: 0
机构:
IREC CSIC UCLM JCCM, SaBio, Inst Invest Recursos Cineget, Ciudad Real 13005, Spain
Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathol, Stillwater, OK 74078 USAInst Higiene & Med Trop, P-1349008 Lisbon, Portugal
机构:
E AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYAE AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYA
BROWN, CGD
;
STAGG, DA
论文数: 0引用数: 0
h-index: 0
机构:
E AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYAE AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYA
STAGG, DA
;
PURNELL, RE
论文数: 0引用数: 0
h-index: 0
机构:
E AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYAE AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYA
PURNELL, RE
;
KANHAI, GK
论文数: 0引用数: 0
h-index: 0
机构:
E AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYAE AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYA
KANHAI, GK
;
PAYNE, RC
论文数: 0引用数: 0
h-index: 0
机构:
E AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYAE AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYA
机构:
Univ Ljubljana, Fac Med, Inst Microbiol & Immunol, Ljubljana 1000, SloveniaUniv Ljubljana, Fac Med, Inst Microbiol & Immunol, Ljubljana 1000, Slovenia
Duh, Darja
;
Punda-Polic, Volga
论文数: 0引用数: 0
h-index: 0
机构:
Univ Hosp, Split 21000, Croatia
Sch Med Split, Split 21000, CroatiaUniv Ljubljana, Fac Med, Inst Microbiol & Immunol, Ljubljana 1000, Slovenia
Punda-Polic, Volga
;
Trilar, Tomi
论文数: 0引用数: 0
h-index: 0
机构:
Slovenian Museum Nat Hist, Ljubljana 1000, SloveniaUniv Ljubljana, Fac Med, Inst Microbiol & Immunol, Ljubljana 1000, Slovenia
Trilar, Tomi
;
Avsic-Zupanc, Tatjana
论文数: 0引用数: 0
h-index: 0
机构:
Univ Ljubljana, Fac Med, Inst Microbiol & Immunol, Ljubljana 1000, SloveniaUniv Ljubljana, Fac Med, Inst Microbiol & Immunol, Ljubljana 1000, Slovenia
机构:
IREC CSIC UCLM JCCM, SaBio, Inst Invest Recursos Cineget, Ciudad Real 13005, SpainInst Higiene & Med Trop, P-1349008 Lisbon, Portugal
Perez de la Lastra, Jose M.
;
Alberdi, Pilar
论文数: 0引用数: 0
h-index: 0
机构:
IREC CSIC UCLM JCCM, SaBio, Inst Invest Recursos Cineget, Ciudad Real 13005, SpainInst Higiene & Med Trop, P-1349008 Lisbon, Portugal
Alberdi, Pilar
;
Domingos, Ana
论文数: 0引用数: 0
h-index: 0
机构:
Inst Higiene & Med Trop, Ctr Malaria & Outras Doencas Tropicais, P-1349008 Lisbon, PortugalInst Higiene & Med Trop, P-1349008 Lisbon, Portugal
Domingos, Ana
;
de la Fuente, Jose
论文数: 0引用数: 0
h-index: 0
机构:
IREC CSIC UCLM JCCM, SaBio, Inst Invest Recursos Cineget, Ciudad Real 13005, Spain
Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathol, Stillwater, OK 74078 USAInst Higiene & Med Trop, P-1349008 Lisbon, Portugal
机构:
E AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYAE AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYA
BROWN, CGD
;
STAGG, DA
论文数: 0引用数: 0
h-index: 0
机构:
E AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYAE AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYA
STAGG, DA
;
PURNELL, RE
论文数: 0引用数: 0
h-index: 0
机构:
E AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYAE AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYA
PURNELL, RE
;
KANHAI, GK
论文数: 0引用数: 0
h-index: 0
机构:
E AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYAE AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYA
KANHAI, GK
;
PAYNE, RC
论文数: 0引用数: 0
h-index: 0
机构:
E AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYAE AFRICAN VET RES ORG, UNDP FAO TICK BORNE DIS PROJECT, PO BOX 32, MUGUGA, KENYA
机构:
Univ Ljubljana, Fac Med, Inst Microbiol & Immunol, Ljubljana 1000, SloveniaUniv Ljubljana, Fac Med, Inst Microbiol & Immunol, Ljubljana 1000, Slovenia
Duh, Darja
;
Punda-Polic, Volga
论文数: 0引用数: 0
h-index: 0
机构:
Univ Hosp, Split 21000, Croatia
Sch Med Split, Split 21000, CroatiaUniv Ljubljana, Fac Med, Inst Microbiol & Immunol, Ljubljana 1000, Slovenia
Punda-Polic, Volga
;
Trilar, Tomi
论文数: 0引用数: 0
h-index: 0
机构:
Slovenian Museum Nat Hist, Ljubljana 1000, SloveniaUniv Ljubljana, Fac Med, Inst Microbiol & Immunol, Ljubljana 1000, Slovenia
Trilar, Tomi
;
Avsic-Zupanc, Tatjana
论文数: 0引用数: 0
h-index: 0
机构:
Univ Ljubljana, Fac Med, Inst Microbiol & Immunol, Ljubljana 1000, SloveniaUniv Ljubljana, Fac Med, Inst Microbiol & Immunol, Ljubljana 1000, Slovenia