Establishment of an Artificial Tick Feeding System to Study Theileria lestoquardi Infection

被引:20
作者
Tajeri, Shahin [1 ]
Razmi, Gholamreza [1 ]
Haghparast, Alireza [1 ,2 ]
机构
[1] Ferdowsi Univ Mashhad, Dept Pathobiol, Fac Vet Med, Mashhad, Iran
[2] Ferdowsi Univ Mashhad, Inst Biotechnol, Vet Biotechnol Res Grp, Mashhad, Iran
来源
PLOS ONE | 2016年 / 11卷 / 12期
关键词
HYALOMMA-ANATOLICUM-ANATOLICUM; IN-VITRO; RHIPICEPHALUS-APPENDICULATUS; IXODID TICKS; HARD TICKS; PARVA; TRANSMISSION; BLOOD; SHEEP; DIVERSITY;
D O I
10.1371/journal.pone.0169053
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The establishment of good experimental models for Theileria sp. infection is important for theileriosis research. Routinely, infection of ticks is accomplished by feeding on parasite-infected animals (sheep, cows and horses), which raises practical and ethical problems, driving the search for alternative methods of tick infection. Artificial tick feeding systems are based mainly on rearing ticks on host-derived or hand-made artificial membranes. We developed a modified feeding assay for infecting nymphal stages of Hyalomma anatolicum ticks with Theileria lestoquardi, a highly pathogenic parasite of sheep. We compared two different membranes: an artificial silicone membrane and a natural alternative using mouse skin. We observed high attachment rates with mouse skin, whereas in vitro feeding of H. anatolicum nymphs on silicone membranes was unsuccessful. We could infect H. anatolicum nymphs with T. lestoquardi and the emerging adult ticks transmitted infective parasites to sheep. In contrast, similar infections with Rhipicephalus bursa, a representative tick with short mouth-parts that was proposed as a vector for T. lestoquardi, appeared not to be a competent vector tick species. This is the first report of an experimentally controlled infection of H. anatolicum with T. lestoquardi and opens avenues to explore tick-parasite dynamics in detail.
引用
收藏
页数:14
相关论文
共 44 条
[1]   Molecular and serological detection of Theileria equi and Babesia caballi infection in horses and ixodid ticks in Iran [J].
Abedi, Vali ;
Razmi, Golamreza ;
Seifi, Hesam ;
Naghibi, Abolghasem .
TICKS AND TICK-BORNE DISEASES, 2014, 5 (03) :239-244
[2]   PCR-based detection of Theileria ovis in Rhipicephalus bursa adult ticks [J].
Aktas, M. ;
Altay, K. ;
Dumanli, N. .
VETERINARY PARASITOLOGY, 2006, 140 (3-4) :259-263
[3]   A Silicone Membrane for In Vitro Feeding of Ixodes scapularis (Ixodida: Ixodidae) [J].
Andrade, John J. ;
Xu, Guang ;
Rich, Stephen M. .
JOURNAL OF MEDICAL ENTOMOLOGY, 2014, 51 (04) :878-879
[4]   Tick capillary feeding for the study of proteins involved in tick-pathogen interactions as potential antigens for the control of tick infestation and pathogen infection [J].
Antunes, Sandra ;
Merino, Octavio ;
Mosqueda, Juan ;
Moreno-Cid, Juan A. ;
Bell-Sakyi, Lesley ;
Fragkoudis, Rennos ;
Weisheit, Sabine ;
Perez de la Lastra, Jose M. ;
Alberdi, Pilar ;
Domingos, Ana ;
de la Fuente, Jose .
PARASITES & VECTORS, 2014, 7
[5]   Incidence and prevalence of tick-borne haemoparasites in domestic ruminants in Ghana [J].
Bell-Sakyi, L ;
Koney, EBM ;
Dogbey, O ;
Walker, AR .
VETERINARY PARASITOLOGY, 2004, 124 (1-2) :25-42
[6]   Transstadial and transovarial persistence of Babesia divergens DNA in Ixodes ricinus ticks fed on infected blood in a new skin-feeding technique [J].
Bonnet, S. ;
Jouglin, M. ;
Malandrin, L. ;
Becker, C. ;
Agoulon, A. ;
L'Hostis, M. ;
Chauvin, A. .
PARASITOLOGY, 2007, 134 :197-207
[7]   LABORATORY ARTIFICIAL INFECTION OF HARD TICKS: A TOOL FOR THE ANALYSIS OF TICK-BORNE PATHOGEN TRANSMISSION [J].
Bonnet, Sarah ;
Liu, Xiang Ye .
ACAROLOGIA, 2012, 52 (04) :453-464
[8]   INFECTION AND TRANSFORMATION OF BOVINE LYMPHOID-CELLS IN-VITRO BY INFECTIVE PARTICLES OF THEILERIA-PARVA [J].
BROWN, CGD ;
STAGG, DA ;
PURNELL, RE ;
KANHAI, GK ;
PAYNE, RC .
NATURE, 1973, 245 (5420) :101-103
[9]  
Dschunkovsky E., 1924, Parasitology Cambridge, V16, P107, DOI 10.1017/S0031182000019922
[10]   Molecular detection of Theileria sp in ticks and naturally infected sheep [J].
Duh, Darja ;
Punda-Polic, Volga ;
Trilar, Tomi ;
Avsic-Zupanc, Tatjana .
VETERINARY PARASITOLOGY, 2008, 151 (2-4) :327-331