Synergistic response of physicochemical reaction parameters on biogenesis of silver nanoparticles and their action against colon cancer and leishmanial cells

Physicochemical parameters include pH, temperature, the concentration of the AgNO3, ratio of reactants, agitation and incubation period that act synergistically and provide a steering force to modulate the biogenesis of nanoparticles by influencing the molecular dynamics, reaction kinetics, protein conformations, and catalysis. The current study involved the bio-fabrication of silver nanoparticles (SNPs) by using the reducing abilities of Mentha longifolia (L.) L. leaves aqueous extract. Spectrophotometric analysis of various biochemical reactions showed that 3 mM of AgNO3 at 120 degrees C in an acidic pH when mixed in 1-9 ratio of plant extract and AgNO3 respectively, are the optimised conditions for SNPs synthesis. Different analytical techniques confirmed that the nanoparticles are anisotropic and nearly spherical and have a size range of 10-100 nm. The similar to 10 mu g/ml of SNPs killed similar to 66% of Leishmania population and IC50 was measured at 8.73 mu g/ml. SRB assay and Annexin V apoptosis assay results showed that the plant aqueous extract and SNPs are not active against HCT116 colon cancer cells and no IC50 (80% survival) was reported. ROS generation was quantified at 0.08 phi, revealed that the SNPs from M. longifolia can generate free radicals and no photothermal activity was recorded which makes them non-photodynamic.