Kinetics of total enzymatic hydrolysis of acetylcholine and acetylthiocholine

被引：0
|
作者：
Zdrazilova, Pavla
Stepankova, Sarka
Vranova, Martina
Komers, Karel
Komersova, Alena
Cegan, Alexander
机构：
[1] Univ Pardubice, Dept Phys Chem, Fac Chem Technol, Pardubice 53210, Czech Republic
[2] Univ Pardubice, Dept Biol & Biochem Sci, Pardubice 53002, Czech Republic
来源：
ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES | 2006年 / 61卷 / 3-4期
关键词：
acetylcholine; hydrolysis; kinetics;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Kinetics and the mechanism of total in vitro hydrolyses (i.e. up to the exhaustion of substrate) of acetylcholine and acetylthiocholine by acetylcholinesterase and butyrylcholinesterase were studied in vitro in a batch reactor at 25 degrees C, pH 8 and ionic strength of 0.11 M. Every hydrolysis was monitored by 2-3 independent analytical methods. All studied types of enzymatic hydrolyses fulfilled the Michaelis-Menten reaction scheme with the irreversible second step. A table of obtained average values of rate constants and estimations of initial molar enzyme concentrations, and discussion of the results are presented.
引用
收藏
页码：289 / 294
页数：6
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