Determination of thujone by derivatization with dansylhydrazine and liquid chromatography

被引：9
|
作者：
Scott, PM [1 ]
Lawrence, GA [1 ]
Lau, BPY [1 ]
机构：
[1] Hlth Canada, Hlth Prod & Food Branch, Food Res Div, Ottawa, ON K1A 0L2, Canada
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2004年 / 27卷 / 06期
关键词：
liquid chromatography; dansylhydrazine; thujone; vermouth;
D O I：
10.1081/JLC-120030179
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The reaction of alpha- and beta-thujones with dansylhydrazine (DNSH) was studied using reversed phase liquid chromatography (LC) with fluorescence detection. The derivatization procedure, although slow, was applied for determination of alpha-thujone in white vermouth at concentrations 0.45-4.5 mug/L using a C-18 solid phase extraction column for cleanup; recoveries of added a-thujone were in the range 60-77%. Separation of dansyl alpha- and beta-thujones was achieved by LC and their identity confirmed by LC-tandem mass spectrometry (MS/MS).
引用
收藏
页码：1071 / 1081
页数：11
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