Citrus Flavanones Upregulate Thyrotroph Sirt1 and Differently Affect Thyroid Nrf2 Expressions in Old-Aged Wistar Rats

被引：21

作者：

Miler, Marko ^{[1
]}

Zivanovic, Jasmina ^{[1
]}

Ajdzanovic, Vladimir ^{[1
]}

Milenkovic, Dragan ^{[2
,3
]}

Jaric, Ivana ^{[1
,4
]}

Sosic-Jurjevic, Branka ^{[1
]}

Milosevic, Verica ^{[1
]}

机构：

[1] Univ Belgrade, Dept Cytol, Inst Biol Res Sinisa Stankovic, Natl Inst Republ Serbia, Belgrade 11060, Serbia

[2] Univ Clermont Auvergne, INRAE, UNH, F-63000 Clermont Ferrand, France

[3] Univ Calif Davis, Div Cardiovasc Med, Davis, CA 95616 USA

[4] Univ Bern, Anim Welf Div, Vetsuisse, CH-3012 Bern, Switzerland

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2020年 / 68卷 / 31期

关键词：

naringenin; hesperetin; thyroid ultrastructure; thyroid peroxidase; antioxidant enzymes; ANTIOXIDANT DEFENSE; STRESS; THYROPEROXIDASE; TRANSCRIPTION; IMPAIRMENT; FLAVONOIDS; LONGEVITY; RESPONSES; PATHWAY; SERUM;

D O I：

10.1021/acs.jafc.0c03079

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A growing population of elderly people consume citrus flavanones, naringenin, and hesperetin in the form of fruits or juices. Flavanones are bioactives with potent antioxidant properties and have potential in slowing down the aging process. Because flavanones exert controversial effects on pituitary-thyroid functioning, our study on the old-aged rat model aimed to elucidate the mechanism by which naringenin and hesperetin affect this axis. Naringenin and hesperetin increased the Sirt1 mRNA level by 91 and 71% (p < 0.05), which was followed by increased Sirt1 expression by 20 and 15% (p < 0.05), respectively. Only naringenin decreased thyroid-stimulating hormone expression by 20% (p < 0.05). Thyroid peroxidase protein expression was upregulated after naringenin or hesperetin by 62 and 43% (p < 0.05), respectively. Naringenin lowered mRNA levels of Tpo, Sod1, Sod2, Cat, and Nrf 2 by 50, 32, 45, 35, and 42% (p < 0.05), respectively, and increased Gpx by 54% (p < 0.05), while hesperetin decreased Sod1 and Sod2 mRNA levels by 46 and 55% (p < 0.05), respectively. Naringenin increased the protein expressions of Nrf2 and SOD2 by 58 and 50% (p < 0.05), respectively, and decreased SOD1 expression by 48% (p < 0.05), while hesperetin protein decreased expressions of SOD1 and Nrf2 by 63 and 32% (p < 0.05), respectively. Altogether, our findings suggest that citrus flavanones contribute to restoring the impaired thyroid functioning in the old-aged rats.

引用

页码：8242 / 8254

页数：13

共 53 条

[31] The Nrf2-Antioxidant Response Element Signaling Pathway and Its Activation by Oxidative Stress [J].