Fatty acid composition and squalene content of the marine microalga Schizochytrium mangrovei

被引:125
作者
Jiang, Y [1 ]
Fan, KW
Wong, RDY
Chen, F
机构
[1] Hong Kong Baptist Univ, Dept Biol, Kowloon, Hong Kong, Peoples R China
[2] Univ Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China
关键词
fatty acids; docosahexaenoic acid; DHA; squalene; Schizochytrium mangrovei;
D O I
10.1021/jf035004c
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Certain species of thraustochytrids are being explored as potential producer of polyunsaturated fatty acids for nutritional enrichment of food products and use as feed additives in aquaculture. The fatty acid composition and squalene content were determined in the thraustochytrid, Schizochytrium mangrovei that was newly isolated from decaying Kandelia candel leaves in Hong Kong mangrove habitat. The major fatty acid constituents identified in all three S. mangrovei strains were tetradeanoic acid (C14:0), hexadecanoic acid (C16:0), docosapentaenoic acid (C22:5 n-6, DPA) and docosahexaenoic acid (C22:6 n-3, DHA). DHA was the most predominant polyunsaturated fatty acid, and the percentage of DHA (of total fatty acids) in all these strains varied from 32.29 to 39.14%. Only slight changes were observed in fatty acid composition of the S. mangrovei strains harvested at their early (day 3) and late stationary (day 5) phases. In contrast, the cellular squalene content was affected significantly by the culture time; the largest decrease of squalene content from 0.162 mg/g to 0.035 mg/g was found in S. mangrovei FB1 as the culture aged.
引用
收藏
页码:1196 / 1200
页数:5
相关论文
共 30 条
[21]   REGULATION OF SQUALENE SYNTHETASE AND SQUALENE EPOXIDASE ACTIVITIES IN SACCHAROMYCES-CEREVISIAE [J].
MBAYA, B ;
FEGUEUR, M ;
SERVOUSE, M ;
KARST, F .
LIPIDS, 1989, 24 (12) :1020-1023
[22]   USE OF DINITROSALICYLIC ACID REAGENT FOR DETERMINATION OF REDUCING SUGAR [J].
MILLER, GL .
ANALYTICAL CHEMISTRY, 1959, 31 (03) :426-428
[23]   Determination of squalene in olive oil using fractional crystallization for sample preparation [J].
Nenadis, N ;
Tsimidou, M .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 2002, 79 (03) :257-259
[24]   Chemopreventive effect of squalene on colon cancer [J].
Rao, CV ;
Newmark, HL ;
Reddy, BS .
CARCINOGENESIS, 1998, 19 (02) :287-290
[25]   Docosahexaenoic acid (DHA) production by Thraustochytrium sp ATCC 20892 [J].
Singh, A ;
Wilson, S ;
Ward, OP .
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1996, 12 (01) :76-81
[26]   Squalene: potential chemopreventive agent [J].
Smith, TJ .
EXPERT OPINION ON INVESTIGATIONAL DRUGS, 2000, 9 (08) :1841-1848
[27]   Changes in the lipid composition of Saccharomyces cerevisiae race Capensis (G-1) during alcoholic fermentation and flor film formation [J].
Valero, E ;
Millán, C ;
Ortega, JM .
LEBENSMITTEL-WISSENSCHAFT UND-TECHNOLOGIE-FOOD SCIENCE AND TECHNOLOGY, 2002, 35 (07) :593-599
[28]   Lipids and ultrastructure of Thraustochytrium sp. ATCC 26185 [J].
Weete, JD ;
Kim, H ;
Gandhi, SR ;
Wang, Y ;
Dute, R .
LIPIDS, 1997, 32 (08) :839-845
[29]   Optimization of docosahexaenoic acid production by Schizochytrium limacinum SR21 [J].
Yokochi, T ;
Honda, D ;
Higashihara, T ;
Nakahara, T .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1998, 49 (01) :72-76
[30]  
YOKOCHI T, 1999, 4 INT MAR BIOT C IT, P263