A guide to microRNA-mediated gene silencing

被引：48
|
作者：
Huberdeau, Miguel Quevillon [1 ,2 ]
Simard, Martin J. [1 ,2 ]
机构：
[1] Univ Laval, Res Ctr, CHU Quebec, Oncol Div,St Patrick Res Grp Basic Oncol, Quebec City, PQ G1R 3S3, Canada
[2] Laval Univ, Canc Res Ctr, Quebec City, PQ, Canada
来源：
FEBS JOURNAL | 2019年 / 286卷 / 04期
基金：
加拿大健康研究院; 加拿大自然科学与工程研究理事会;
关键词：
gene regulation; microRNA target; microRNA target validation; miRISC; RNA-BINDING PROTEIN; SEQUENCE-SPECIFIC INHIBITION; ARGONAUTE PROTEINS; TARGET SITES; IDENTIFICATION; PROTEOMICS; REVEALS; HUR; DETERMINANTS; TRANSLATION;
D O I：
10.1111/febs.14666
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
As an important regulator of gene expression in eukaryotes, microRNAs (miRNAs) have been subject to extensive research to understand their pleiotropic roles in development and pathological processes. Here, we review strategies that have been developed to map microRNA-target interactions and the most effective methods to attribute post-transcriptional gene regulation through miRNA-mediated silencing. We further discuss the importance of the biological context for silencing.
引用
收藏
页码：642 / 652
页数：11
相关论文
共 50 条
  • [21] A continuum of mRNP complexes in embryonic microRNA-mediated silencing
    Wu, Edlyn
    Vashisht, Ajay A.
    Chapat, Clement
    Flamand, Mathieu N.
    Cohen, Emiliano
    Sarov, Mihail
    Tabach, Yuval
    Sonenberg, Nahum
    Wohlschlegel, James
    Duchaine, Thomas F.
    NUCLEIC ACIDS RESEARCH, 2017, 45 (04) : 2081 - 2098
  • [22] An in vivo approach to the discovery and characterization of cellular mechanisms regulating microRNA-mediated gene silencing
    Browning, Austin
    Rees, Katherina
    Depner, Mary
    Sadino, Julie
    Reinke, Catherine
    FASEB JOURNAL, 2014, 28 (01):
  • [23] Mechanisms of microRNA-mediated gene regulation
    Shuang Zhao
    Mo-Fang Liu
    Science in China Series C: Life Sciences, 2009, 52 : 1111 - 1116
  • [24] Mechanisms of microRNA-mediated gene regulation
    Nilsen, Timothy W.
    FASEB JOURNAL, 2007, 21 (05): : A92 - A92
  • [25] NON-CODING RNA Towards a molecular understanding of microRNA-mediated gene silencing
    Jonas, Stefanie
    Izaurralde, Elisa
    NATURE REVIEWS GENETICS, 2015, 16 (07) : 421 - 433
  • [26] Mechanisms of microRNA-mediated gene regulation
    ZHAO Shuang & LIU Mo-Fang· State Key Laboratory of Molecular Biology
    Science in China(Series C:Life Sciences), 2009, (12) : 1111 - 1116
  • [27] Mechanisms of microRNA-mediated gene regulation
    Zhao Shuang
    Liu Mo-Fang
    SCIENCE IN CHINA SERIES C-LIFE SCIENCES, 2009, 52 (12): : 1111 - 1116
  • [28] MicroRNA 665 Regulates Dentinogenesis through MicroRNA-Mediated Silencing and Epigenetic Mechanisms
    Heair, Hannah M.
    Kemper, Austin G.
    Roy, Bhaskar
    Lopes, Helena B.
    Rashid, Harunur
    Clarke, John C.
    Afreen, Lubana K.
    Ferraz, Emanuela P.
    Kim, Eddy
    Javed, Amjad
    Beloti, Marcio M.
    MacDougall, Mary
    Hassana, Mohammad Q.
    MOLECULAR AND CELLULAR BIOLOGY, 2015, 35 (18) : 3116 - 3130
  • [29] A computational tool for the design of live attenuated virus vaccine based on microRNA-mediated gene silencing
    Wichadakul, Duangdao
    Mhuantong, Wuttichai
    Jongkaewwattana, Anan
    Ingsriswang, Supawadee
    BMC GENOMICS, 2012, 13
  • [30] MicroRNA-mediated gene silencing modulates the UV-induced DNA-damage response
    Pothof, Joris
    Verkaik, Nicole S.
    van IJcken, Wilfred
    Wiemer, Erik A. C.
    Ta, Van T. B.
    van der Horst, Gijsbertus T. J.
    Jaspers, Nicolaas G. J.
    van Gent, Dik C.
    Hoeijmakers, Jan H. J.
    Persengiev, Stephan P.
    EMBO JOURNAL, 2009, 28 (14): : 2090 - 2099
12345