A guide to microRNA-mediated gene silencing

被引：51

作者：

Huberdeau, Miguel Quevillon ^{[1
,2
]}

Simard, Martin J. ^{[1
,2
]}

机构：

[1] Univ Laval, Res Ctr, CHU Quebec, Oncol Div,St Patrick Res Grp Basic Oncol, Quebec City, PQ G1R 3S3, Canada

[2] Laval Univ, Canc Res Ctr, Quebec City, PQ, Canada

来源：

FEBS JOURNAL | 2019年 / 286卷 / 04期

基金：

加拿大健康研究院; 加拿大自然科学与工程研究理事会;

关键词：

gene regulation; microRNA target; microRNA target validation; miRISC; RNA-BINDING PROTEIN; SEQUENCE-SPECIFIC INHIBITION; ARGONAUTE PROTEINS; TARGET SITES; IDENTIFICATION; PROTEOMICS; REVEALS; HUR; DETERMINANTS; TRANSLATION;

D O I：

10.1111/febs.14666

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

As an important regulator of gene expression in eukaryotes, microRNAs (miRNAs) have been subject to extensive research to understand their pleiotropic roles in development and pathological processes. Here, we review strategies that have been developed to map microRNA-target interactions and the most effective methods to attribute post-transcriptional gene regulation through miRNA-mediated silencing. We further discuss the importance of the biological context for silencing.

引用

页码：642 / 652

页数：11

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