Effect of Dietary Carbohydrate-to-Protein Ratio on Gut Microbiota in Atlantic Salmon (Salmo salar)

被引:34
作者
Villasante, Alejandro [1 ]
Ramirez, Carolina [1 ]
Catalan, Natalia [1 ]
Opazo, Rafael [1 ]
Dantagnan, Patricio [2 ]
Romero, Jaime [1 ]
机构
[1] Univ Chile, INTA, Lab Biotecnol Alimentos, Unidad Alimentos, Santiago 7830490, Chile
[2] Univ Catolica Temuco, Dept Ciencias Agr & Acuicolas, Fac Recursos Nat, Nucleo Invest Prod Alimentaria, Temuco 4781312, Chile
来源
ANIMALS | 2019年 / 9卷 / 03期
关键词
Atlantic salmon (Salmo salar); microbiota; carbohydrates; carbohydrate/protein ratio; TROUT ONCORHYNCHUS-MYKISS; RAINBOW-TROUT; GLUCOSE-METABOLISM; INTESTINAL MICROBIOTA; FISH; GROWTH; DIVERSITY;
D O I
10.3390/ani9030089
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Atlantic salmon (Salmo salar) is a carnivorous fish species whose productive performance tends to be suboptimal when fed low-cost carbohydrate rich meals. It is of interest to study the dynamics of gut microbiota communities in salmonids fed high carbohydrate diets since gut microbes are referred to as key players that influence the metabolism and physiology of the host. A study was conducted to determine the effect of feeding a high carbohydrate diet to Atlantic salmon in gut microbiota communities. A medium carbohydrate (15% wheat starch)/medium protein (MC/MP) diet or a high carbohydrate (30% wheat starch)/low protein (HC/LP) diet was fed to triplicate tanks (28 fish each) during four weeks. We conducted an in-depth characterization of the distal intestine digesta microbiota using high-throughput sequencing of the V4 region of the 16S rRNA gene. Firmicutes, Actinobacteria and Proteobacteria were the major phyla determined in either experimental group. Phylum Planctomycetes, class Planctomycetia, order Planctomycetales and genus Lactococcus were significantly more abundant in fish fed the HC/LP diet compared with fish fed the MC/MP diet. Our study suggests feeding a carbohydrate rich meal to salmon exerts a low impact on the structure of gut microbial communities, affecting mostly low-abundance bacteria capable of metabolizing anaerobically carbohydrates as a major energy-yielding substrate.
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页数:16
相关论文
共 48 条
  • [1] Dietary synbiotic application modulates Atlantic salmon (Salmo salar) intestinal microbial communities and intestinal immunity
    Abid, A.
    Davies, S. J.
    Waines, R.
    Emery, M.
    Castex, M.
    Gioacchini, G.
    Carnevali, O.
    Bickerdike, R.
    Romero, J.
    Merrifield, D. L.
    [J]. FISH & SHELLFISH IMMUNOLOGY, 2013, 35 (06) : 1948 - 1956
  • [2] [Anonymous], 2011, Nutrient Requirements of Fish and Shrimp, P376, DOI DOI 10.17226/13039
  • [3] Determining the bacterial cell biology of Planctomycetes
    Boedeker, Christian
    Schueler, Margarete
    Reintjes, Greta
    Jeske, Olga
    van Teeseling, Muriel C. F.
    Jogler, Mareike
    Rast, Patrick
    Borchert, Daniela
    Devos, Damien P.
    Kucklick, Martin
    Schaffer, Miroslava
    Kolter, Roberto
    van Niftrik, Laura
    Engelmann, Susanne
    Amann, Rudolf
    Rohde, Manfred
    Engelhardt, Harald
    Jogler, Christian
    [J]. NATURE COMMUNICATIONS, 2017, 8
  • [4] Gut histology, immunology and the intestinal microbiota of rainbow trout, Oncorhynchus mykiss (Walbaum), fed process variants of soybean meal
    Bruce, Timothy J.
    Neiger, Regg D.
    Brown, Michael L.
    [J]. AQUACULTURE RESEARCH, 2018, 49 (01) : 492 - 504
  • [5] Callahan BJ, 2016, NAT METHODS, V13, P581, DOI [10.1038/nmeth.3869, 10.1038/NMETH.3869]
  • [6] QIIME allows analysis of high-throughput community sequencing data
    Caporaso, J. Gregory
    Kuczynski, Justin
    Stombaugh, Jesse
    Bittinger, Kyle
    Bushman, Frederic D.
    Costello, Elizabeth K.
    Fierer, Noah
    Pena, Antonio Gonzalez
    Goodrich, Julia K.
    Gordon, Jeffrey I.
    Huttley, Gavin A.
    Kelley, Scott T.
    Knights, Dan
    Koenig, Jeremy E.
    Ley, Ruth E.
    Lozupone, Catherine A.
    McDonald, Daniel
    Muegge, Brian D.
    Pirrung, Meg
    Reeder, Jens
    Sevinsky, Joel R.
    Tumbaugh, Peter J.
    Walters, William A.
    Widmann, Jeremy
    Yatsunenko, Tanya
    Zaneveld, Jesse
    Knight, Rob
    [J]. NATURE METHODS, 2010, 7 (05) : 335 - 336
  • [7] Environmental and physiological factors shape the gut microbiota of Atlantic salmon parr (Salmo salar L.)
    Dehler, Carola E.
    Secombes, Christopher J.
    Martin, Samuel A. M.
    [J]. AQUACULTURE, 2017, 467 : 149 - 157
  • [8] Effects of plant-based diets on the distal gut microbiome of rainbow trout (Oncorhynchus mykiss)
    Desai, Atul R.
    Links, Matthew G.
    Collins, Stephanie A.
    Mansfield, Graeme S.
    Drew, Murray D.
    Van Kessel, Andrew G.
    Hill, Janet E.
    [J]. AQUACULTURE, 2012, 350 : 134 - 142
  • [9] Dietary mannan oligosaccharide supplementation modulates intestinal microbial ecology and improves gut morphology of rainbow trout, Oncorhynchus mykiss (Walbaum)
    Dimitroglou, A.
    Merrifield, D. L.
    Moate, R.
    Davies, S. J.
    Spring, P.
    Sweetman, J.
    Bradley, G.
    [J]. JOURNAL OF ANIMAL SCIENCE, 2009, 87 (10) : 3226 - 3234
  • [10] Dworkin M., 2006, PROKARYOTES HDB BIOL, V1, P137