Bifunctional fluorescent probes for detection of amyloid aggregates and reactive oxygen species

被引：13

作者：

Needham, Lisa-Maria ^{[1
]}

Weber, Judith ^{[2
,3
]}

Fyfe, James W. B. ^{[4
]}

Kabia, Omaru M. ^{[4
]}

Do, Dung T. ^{[4
]}

Klimont, Ewa ^{[1
]}

Zhang, Yu ^{[1
]}

Rodrigues, Margarida ^{[1
]}

Dobson, Christopher M. ^{[1
]}

Ghandi, Sonia ^{[5
]}

Bohndiek, Sarah E. ^{[2
,3
]}

Snaddon, Thomas N. ^{[4
]}

Lee, Steven F. ^{[1
]}

机构：

[1] Univ Cambridge, Dept Chem, Lensfield Rd, Cambridge CB2 1EW, England

[2] Univ Cambridge, Dept Phys, JJ Thomson Ave, Cambridge CB3 0HE, England

[3] Univ Cambridge, Li Ka Shing Ctr, Canc Res UK Cambridge Inst, Robinson Way, Cambridge CB2 0RE, England

[4] Indiana Univ, Dept Chem, 800 E Kirkwood Ave, Bloomington, IN 47405 USA

[5] UCL, Inst Neurol, Dept Mol Neurosci, Queen Sq, London WC1N 3BG, England

来源：

ROYAL SOCIETY OPEN SCIENCE | 2018年 / 5卷 / 02期

关键词：

fluorophores; ROS; sensors; protein aggregation; single-molecule imaging; Thioflavin T; HYDROGEN-PEROXIDE; THIOFLAVIN-T; OXIDATIVE STRESS; MITOCHONDRIAL DYSFUNCTION; PEPTIDE; BINDING;

D O I：

10.1098/rsos.171399

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Protein aggregation into amyloid deposits and oxidative stress are key features of many neurodegenerative disorders including Parkinson's and Alzheimer's disease. We report here the creation of four highly sensitive bifunctional fluorescent probes, capable of H2O2 and/or amyloid aggregate detection. These bifunctional sensors use a benzothiazole core for amyloid localization and boronic ester oxidation to specifically detect H2O2. We characterized the optical properties of these probes using both bulk fluorescence measurements and single-aggregate fluorescence imaging, and quantify changes in their fluorescence properties upon addition of amyloid aggregates of alpha-synuclein and pathophysiological H2O2 concentrations. Our results indicate these new probes will be useful to detect and monitor neurodegenerative disease.

引用

页数：12

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