Modeling Disease In Vivo With CRISPR/Cas9

被引：92

作者：

Dow, Lukas E. ^{[1
]}

机构：

[1] Weill Cornell Med Coll, Dept Med Hematol & Med Oncol, New York, NY 10021 USA

来源：

TRENDS IN MOLECULAR MEDICINE | 2015年 / 21卷 / 10期

基金：

美国国家卫生研究院;

关键词：

ONE-STEP GENERATION; CAENORHABDITIS-ELEGANS GENOME; HOMOLOGY-DIRECTED REPAIR; OFF-TARGET MUTATIONS; STRAND-BREAK REPAIR; CRISPR-CAS9; SYSTEM; MUSCULAR-DYSTROPHY; HUMAN-CELLS; CHROMOSOMAL REARRANGEMENTS; EDITING SPECIFICITY;

D O I：

10.1016/j.molmed.2015.07.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The recent advent of CRISPR/Cas9-mediated genome editing has created a wave of excitement across the scientific research community, carrying the promise of simple and effective genomic manipulation of nearly any cell type. CRISPR has quickly become the preferred tool for genetic manipulation, and shows incredible promise as a platform for studying gene function in vivo. I discuss the current application of CRISPR technology to create new in vivo disease models, with a particular focus on how these tools, derived from an adaptive bacterial immune system, are helping us to better model the complexity of human cancer.

引用

页码：609 / 621

页数：13

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