Modeling Disease In Vivo With CRISPR/Cas9

被引：90
作者：
Dow, Lukas E. [1 ]
机构：
[1] Weill Cornell Med Coll, Dept Med Hematol & Med Oncol, New York, NY 10021 USA
来源：
TRENDS IN MOLECULAR MEDICINE | 2015年 / 21卷 / 10期
基金：
美国国家卫生研究院;
关键词：
ONE-STEP GENERATION; CAENORHABDITIS-ELEGANS GENOME; HOMOLOGY-DIRECTED REPAIR; OFF-TARGET MUTATIONS; STRAND-BREAK REPAIR; CRISPR-CAS9; SYSTEM; MUSCULAR-DYSTROPHY; HUMAN-CELLS; CHROMOSOMAL REARRANGEMENTS; EDITING SPECIFICITY;
D O I：
10.1016/j.molmed.2015.07.006
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The recent advent of CRISPR/Cas9-mediated genome editing has created a wave of excitement across the scientific research community, carrying the promise of simple and effective genomic manipulation of nearly any cell type. CRISPR has quickly become the preferred tool for genetic manipulation, and shows incredible promise as a platform for studying gene function in vivo. I discuss the current application of CRISPR technology to create new in vivo disease models, with a particular focus on how these tools, derived from an adaptive bacterial immune system, are helping us to better model the complexity of human cancer.
引用
收藏
页码：609 / 621
页数：13
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  • [1] A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish
    Ablain, Julien
    Durand, Ellen M.
    Yang, Song
    Zhou, Yi
    Zon, Leonard I.
    [J]. DEVELOPMENTAL CELL, 2015, 32 (06) : 756 - 764
  • [2] Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice
    Aida, Tomomi
    Chiyo, Keiho
    Usami, Takako
    Ishikubo, Harumi
    Imahashi, Risa
    Wada, Yusaku
    Tanaka, Kenji F.
    Sakuma, Tetsushi
    Yamamoto, Takashi
    Tanaka, Kohichi
    [J]. GENOME BIOLOGY, 2015, 16
  • [3] A prudent path forward for genomic engineering and germline gene modification
    Baltimore, David
    Berg, Paul
    Botchan, Michael
    Carroll, Dana
    Charo, R. Alta
    Church, George
    Corn, Jacob E.
    Daley, George Q.
    Doudna, Jennifer A.
    Fenner, Marsha
    Greely, Henry T.
    Jinek, Martin
    Martin, G. Steven
    Penhoet, Edward
    Puck, Jennifer
    Sternberg, Samuel H.
    Weissman, Jonathan S.
    Yamamoto, Keith R.
    [J]. SCIENCE, 2015, 348 (6230) : 36 - 38
  • [4] Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System
    Bassett, Andrew R.
    Tibbit, Charlotte
    Ponting, Chris P.
    Liu, Ji-Long
    [J]. CELL REPORTS, 2013, 4 (01): : 220 - 228
  • [5] Simple and Rapid In Vivo Generation of Chromosomal Rearrangements using CRISPR/Cas9 Technology
    Blasco, Rafael B.
    Karaca, Elif
    Ambrogio, Chiara
    Cheong, Taek-Chin
    Karayol, Emre
    Minero, Valerio G.
    Voena, Claudia
    Chiarle, Roberto
    [J]. CELL REPORTS, 2014, 9 (04): : 1219 - 1227
  • [6] Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin
    Bolotin, A
    Ouinquis, B
    Sorokin, A
    Ehrlich, SD
    [J]. MICROBIOLOGY-SGM, 2005, 151 : 2551 - 2561
  • [7] Protospacer Adjacent Motif (PAM)-Distal Sequences Engage CRISPR Cas9 DNA Target Cleavage
    Cencic, Regina
    Miura, Hisashi
    Malina, Abba
    Robert, Francis
    Ethier, Sylvain
    Schmeing, T. Martin
    Dostie, Josee
    Pelletier, Jerry
    [J]. PLOS ONE, 2014, 9 (10):
  • [8] Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting
    Cermak, Tomas
    Doyle, Erin L.
    Christian, Michelle
    Wang, Li
    Zhang, Yong
    Schmidt, Clarice
    Baller, Joshua A.
    Somia, Nikunj V.
    Bogdanove, Adam J.
    Voytas, Daniel F.
    [J]. NUCLEIC ACIDS RESEARCH, 2011, 39 (12) : e82
  • [9] Targeted Germline Modifications in Rats Using CRISPR/Cas9 and Spermatogonial Stem Cells
    Chapman, Karen M.
    Medrano, Gerardo A.
    Jaichander, Priscilla
    Chaudhary, Jaideep
    Waits, Alexandra E.
    Nobrega, Marcelo A.
    Hotaling, James M.
    Ober, Carole
    Hamra, F. Kent
    [J]. CELL REPORTS, 2015, 10 (11): : 1828 - 1835
  • [10] Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis
    Chen, Sidi
    Sanjana, Neville E.
    Zheng, Kaijie
    Shalem, Ophir
    Lee, Kyungheon
    Shi, Xi
    Scott, David A.
    Song, Jun
    Pan, Jen Q.
    Weissleder, Ralph
    Lee, Hakho
    Zhang, Feng
    Sharp, Phillip A.
    [J]. CELL, 2015, 160 (06) : 1246 - 1260
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