ICP-MS for absolute quantification of proteins for heteroatom-tagged, targeted proteomics

被引:75
作者
Sanz-Medel, Alfredo [1 ]
Montes-Bayon, Maria [1 ]
Bettmer, Joerg [1 ]
Luisa Fernandez-Sanchez, M. [1 ]
Ruiz Encinar, Jorge [1 ]
机构
[1] Univ Oviedo, Fac Chem, Dept Phys & Analyt Chem, E-33006 Oviedo, Spain
关键词
Absolute quantification; Elemental labeling; Heteroatom tag; ICP-MS; Inductively coupled plasma; Laser ablation; Mass spectrometry; Peptide; Protein; Targeted proteomics; PLASMA-MASS SPECTROMETRY; ISOTOPE-DILUTION ANALYSIS; QUANTITATIVE PROTEOMICS; GEL-ELECTROPHORESIS; CAPILLARY-ELECTROPHORESIS; LIQUID-CHROMATOGRAPHY; PROTEASE ASSAYS; URINARY PROTEIN; NANOHPLC-ICPMS; HUMAN SERUM;
D O I
10.1016/j.trac.2012.07.020
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Detection with inductively coupled plasma mass spectrometry (ICP-MS) holds great promise in tackle the challenge of absolute, reliable quantification of proteins and peptides. We identify possible niches in using ICP-MS for MS-based determination (targeted proteomics) of a limited number of proteins. We discuss research selected from the past five years to shed new light on this particular surge in the analytical potential of ICP-MS. We conclude with our assessment of research areas that could attract future analytical activity within this emerging field. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:52 / 63
页数:12
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