Development of polymorphic microsatellite markers issued from pyrosequencing technology for the medicinal mushroom Agaricus subrufescens

被引：11
作者：
Foulongne-Oriol, Marie [1 ]
Spataro, Cathy [1 ]
Moinard, Magalie [1 ]
Cabannes, Delphine [1 ]
Callac, Philippe [1 ]
Savoie, Jean-Michel [1 ]
机构：
[1] INRA, MycSA UR1264, F-33883 Villenave Dornon, France
来源：
FEMS MICROBIOLOGY LETTERS | 2012年 / 334卷 / 02期
关键词：
Agaricus blazei Murrill; almond mushroom; next generation sequencing; simple sequence repeats; MEDIATED ANTITUMOR POLYSACCHARIDES; GENETIC-VARIABILITY; FRUITING BODY; BLAZEI; HIMEMATSUTAKE; ECOLOGISTS; LOCI;
D O I：
10.1111/j.1574-6968.2012.02627.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The recently described procedure of microsatellite-enriched library pyrosequencing was used to isolate microsatellite loci in the gourmet and medicinal mushroom Agaricus subrufescens. Three hundred and five candidate loci containing at least one simple sequence repeats (SSR) locus and for which primers design was successful, were obtained. From a subset of 95 loci, 35 operational and polymorphic SSR markers were developed and characterized on a sample of 14 A.similar to subrufescens genotypes from diverse origins. These SubSSR markers each displayed from two to 10 alleles with an average of 4.66 alleles per locus. The observed heterozygosity ranged from 0 to 0.71. Several multiplex combinations can be set up, making it possible to genotype up to six markers easily and simultaneously. Cross-amplification in some closely congeneric species was successful for a subset of loci. The 35 microsatellite markers developed here provide a highly valuable molecular tool to study genetic diversity and reproductive biology of A.similar to subrufescens.
引用
收藏
页码：119 / 126
页数：8
相关论文
共 34 条
[1]   Twelve microsatellite markers in the invasive tunicate, Didemnum vexillum, isolated from low genome coverage 454 pyrosequencing reads [J].
Abbott, Cathryn L. ;
Ebert, Daniel ;
Tabata, Amy ;
Therriault, Thomas W. .
CONSERVATION GENETICS RESOURCES, 2011, 3 (01) :79-81
[2]   Fast, cost-effective development of species-specific microsatellite markers by genomic sequencing [J].
Abdelkrim, Jawad ;
Robertson, Bruce C. ;
Stanton, Jo-Ann L. ;
Gemmell, Neil J. .
BIOTECHNIQUES, 2009, 46 (03) :185-+
[3]  
Allan G.J., 2010, NATURE ED KNOWLEDGE, V3, P2
[4]   USING THE 454 PYROSEQUENCING-BASED TECHNIQUE IN THE DEVELOPMENT OF NUCLEAR MICROSATELLITE LOCI IN THE ALPINE PLANT ARABIS ALPINA (BRASSICACEAE) [J].
Buehler, Dominique ;
Graf, Rene ;
Holderegger, Rolf ;
Gugerli, Felix .
AMERICAN JOURNAL OF BOTANY, 2011, 98 (05) :E103-E105
[5]   Development of 21 microsatellite markers for the threatened Yarra pygmy perch (Nannoperca obscura) through 454 shot-gun pyrosequencing [J].
Carvalho, Daniel C. ;
Rodriguez-Zarate, Clara J. ;
Hammer, Michael P. ;
Beheregaray, Luciano B. .
CONSERVATION GENETICS RESOURCES, 2011, 3 (04) :601-604
[6]  
Colauto NB, 2002, BRAZ J MICROBIOL, V33, P131, DOI 10.1590/S1517-83822002000200006
[7]   ISOLATION AND CHARACTERIZATION OF MICROSATELLITE LOCI IN RHODODENDRON FERRUGINEUM (ERICACEAE) USING PYROSEQUENCING TECHNOLOGY [J].
Delmas, Chloe E. L. ;
Lhuillier, Emeline ;
Pornon, Andre ;
Escaravage, Nathalie .
AMERICAN JOURNAL OF BOTANY, 2011, 98 (05) :E120-E122
[8]   Challenges of microsatellite isolation in fungi [J].
Dutech, Cyril ;
Enjalbert, Jerome ;
Fournier, Elisabeth ;
Delmotte, Francois ;
Barres, Benoit ;
Carlier, Jean ;
Tharreau, Didier ;
Giraud, Tatiana .
FUNGAL GENETICS AND BIOLOGY, 2007, 44 (10) :933-949
[9]   The medicinal mushroom Agaricus blazei Murrill:: Review of literature and pharmaco-toxicological problems [J].
Firenzuoli, F. ;
Gori, L. ;
Lombardo, G. .
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2008, 5 (01) :3-15
[10]   Novel microsatellite markers suitable for genetic studies in the white button mushroom Agaricus bisporus [J].
Foulongne-Oriol, Marie ;
Spataro, Cathy ;
Savoie, Jean-Michel .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2009, 84 (06) :1125-1135
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