A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma

被引：79
作者：
Guo, Yixing [1 ]
Yang, Xiaofeng [2 ]
Hakuna, Lovemore [1 ]
Barve, Aabha [1 ]
Escobedo, Jorge O. [1 ]
Lowry, Mark [1 ]
Strongin, Robert M. [1 ]
机构：
[1] Portland State Univ, Dept Chem, Portland, OR 97201 USA
[2] NW Univ Xian, Coll Chem & Mat Sci, Inst Analyt Sci, Key Lab Synthet & Nat Funct Mol Chem,Minist Educ, Xian 710069, Peoples R China
来源：
SENSORS | 2012年 / 12卷 / 05期
基金：
美国国家卫生研究院;
关键词：
fluorescent probe; glutathione; micelle-catalyzed; selectivity; BIFUNCTIONAL CHAIN MOLECULES; RING-CLOSURE REACTIONS; LIVING CELLS; FLUORESCENT; CATALYST; CYSTEINE; THIOLS;
D O I：
10.3390/s120505940
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) and homocysteine (Hcy). The proposed method is precise with a relative standard deviation (R.S.D) lower than 6% (n = 3) and has been successfully applied to determine GSH in human plasma with recoveries between 99.2% and 102.3%.
引用
收藏
页码：5940 / 5950
页数：11
相关论文
共 23 条
  • [1] Combinatorial rosamine library and application to in vivo glutathione probe
    Ahn, Young-Hoon
    Lee, Jun-Seok
    Chang, Young-Tae
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (15) : 4510 - +
  • [2] ORIENTATIONAL BINDING OF SUBSTITUTED NAPHTHOATE COUNTERIONS TO THE TETRADECYLTRIMETHYLAMMONIUM BROMIDE MICELLAR INTERFACE
    BACHOFER, SJ
    SIMONIS, U
    NOWICKI, TA
    [J]. JOURNAL OF PHYSICAL CHEMISTRY, 1991, 95 (01) : 480 - 488
  • [3] SYNTHESIS OF SOME STABLE 7-HALO-1,4-THIAZEPINES - POTENTIAL SUBSTITUTED PENAM PRECURSORS
    BLONDEAU, P
    GAUTHIER, R
    BERSE, C
    GRAVEL, D
    [J]. CANADIAN JOURNAL OF CHEMISTRY, 1971, 49 (23): : 3866 - &
  • [4] Fluorescent and colorimetric probes for detection of thiols
    Chen, Xiaoqiang
    Zhou, Ying
    Peng, Xiaojun
    Yoon, Juyoung
    [J]. CHEMICAL SOCIETY REVIEWS, 2010, 39 (06) : 2120 - 2135
  • [5] Regulation of gene expression by reactive oxygen
    Dalton, TD
    Shertzer, HG
    Puga, A
    [J]. ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 1999, 39 : 67 - 101
  • [6] RING-CLOSURE REACTIONS .7. KINETICS AND ACTIVATION PARAMETERS OF LACTONE FORMATION IN RANGE OF 3-MEMBERED TO 23-MEMBERED RINGS
    GALLI, C
    ILLUMINATI, G
    MANDOLINI, L
    TAMBORRA, P
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1977, 99 (08) : 2591 - 2597
  • [7] Galli C, 2000, EUR J ORG CHEM, V2000, P3117
  • [8] Selenium-mediated micellar catalyst: An efficient enzyme model for glutathione peroxidase-like catalysis
    Huang, Xin
    Dong, Zeyuan
    Liu, Junqiu
    Mao, Shizhong
    Xu, Jiayun
    Luo, Guimin
    Shen, Jiacong
    [J]. LANGMUIR, 2007, 23 (03) : 1518 - 1522
  • [9] RING-CLOSURE REACTIONS OF BIFUNCTIONAL CHAIN MOLECULES
    ILLUMINATI, G
    MANDOLINI, L
    [J]. ACCOUNTS OF CHEMICAL RESEARCH, 1981, 14 (04) : 95 - 102
  • [10] Catalyst-free conjugated addition of thiols to α,β-unsaturated carbonyl compounds in water
    Khatik, Gopal L.
    Kumar, Raj
    Chakraborti, Asit K.
    [J]. ORGANIC LETTERS, 2006, 8 (11) : 2432 - 2436
123