Inheritance of Silent rDNA Chromatin Is Mediated by PARP1 via Noncoding RNA

被引：126

作者：

Guetg, Claudio ^{[1
,2
]}

Scheifele, Fabian ^{[1
]}

Rosenthal, Florian ^{[1
,2
]}

Hottiger, Michael O. ^{[1
]}

Santoro, Raffaella ^{[1
]}

机构：

[1] Univ Zurich, Inst Vet Biochem & Mol Biol, CH-8057 Zurich, Switzerland

[2] Univ Zurich, Life Sci Zurich Grad Sch, Mol Life Sci Program, CH-8057 Zurich, Switzerland

来源：

MOLECULAR CELL | 2012年 / 45卷 / 06期

基金：

瑞士国家科学基金会;

关键词：

REMODELING COMPLEX NORC; RIBOSYLATED NUCLEAR PROTEINS; RIBOSOMAL GENE-TRANSCRIPTION; DNA-POLYMERASE-ALPHA; POLY(ADP-RIBOSE) POLYMERASE; HETEROCHROMATIN FORMATION; HISTONE MODIFICATION; REPLICATION FORKS; ADP-RIBOSYLATION; TUMOR-CELLS;

D O I：

10.1016/j.molcel.2012.01.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Faithful propagation of specific chromatin states requires re-establishment of epigenetic marks after every cell division. How the original epigenetic signature is inherited after disruption during DNA replication is still poorly understood. Here, we show that the poly(ADP-ribose)-polymerase-1 (PARP1/ARTD1) is implicated in the maintenance of silent rDNA chromatin during cell division. We demonstrate that PARP1 associates with TIP5, a subunit of the NoRC complex, via the noncoding pRNA and binds to silent rRNA genes after their replication in mid-late S phase. PARP1 represses rRNA transcription and is implicated in the formation of silent rDNA chromatin. Silent rDNA chromatin is a specific substrate for ADP-ribosylation and the enzymatic activity of PARP1 is necessary to establish rDNA silencing. The data unravel a function of PARP1 and ADP-ribosylation that serves to allow for the inheritance of silent chromatin structures, shedding light on how epigenetic marks are transmitted during each cell cycle.

引用

页码：790 / 800

页数：11

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