Inheritance of Silent rDNA Chromatin Is Mediated by PARP1 via Noncoding RNA

被引：126

作者：

Guetg, Claudio ^{[1
,2
]}

Scheifele, Fabian ^{[1
]}

Rosenthal, Florian ^{[1
,2
]}

Hottiger, Michael O. ^{[1
]}

Santoro, Raffaella ^{[1
]}

机构：

[1] Univ Zurich, Inst Vet Biochem & Mol Biol, CH-8057 Zurich, Switzerland

[2] Univ Zurich, Life Sci Zurich Grad Sch, Mol Life Sci Program, CH-8057 Zurich, Switzerland

来源：

MOLECULAR CELL | 2012年 / 45卷 / 06期

基金：

瑞士国家科学基金会;

关键词：

REMODELING COMPLEX NORC; RIBOSYLATED NUCLEAR PROTEINS; RIBOSOMAL GENE-TRANSCRIPTION; DNA-POLYMERASE-ALPHA; POLY(ADP-RIBOSE) POLYMERASE; HETEROCHROMATIN FORMATION; HISTONE MODIFICATION; REPLICATION FORKS; ADP-RIBOSYLATION; TUMOR-CELLS;

D O I：

10.1016/j.molcel.2012.01.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Faithful propagation of specific chromatin states requires re-establishment of epigenetic marks after every cell division. How the original epigenetic signature is inherited after disruption during DNA replication is still poorly understood. Here, we show that the poly(ADP-ribose)-polymerase-1 (PARP1/ARTD1) is implicated in the maintenance of silent rDNA chromatin during cell division. We demonstrate that PARP1 associates with TIP5, a subunit of the NoRC complex, via the noncoding pRNA and binds to silent rRNA genes after their replication in mid-late S phase. PARP1 represses rRNA transcription and is implicated in the formation of silent rDNA chromatin. Silent rDNA chromatin is a specific substrate for ADP-ribosylation and the enzymatic activity of PARP1 is necessary to establish rDNA silencing. The data unravel a function of PARP1 and ADP-ribosylation that serves to allow for the inheritance of silent chromatin structures, shedding light on how epigenetic marks are transmitted during each cell cycle.

引用

页码：790 / 800

页数：11

共 61 条

[1] ISOLATION AND PARTIAL CHARACTERIZATION OF THE ADP-RIBOSYLATED NUCLEAR PROTEINS FROM EHRLICH ASCITES TUMOR-CELLS [J].

ADAMIETZ, P ;

KLAPPROTH, K ;

HILZ, H .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1979, 91 (04) :1232-1238

[2]

ANACHKOVA B, 1989, CYTOBIOS, V59, P19

[3] Rapid regulation of telomere length is mediated by poly(ADP-ribose) polymerase-1 [J].

Beneke, Sascha ;

Cohausz, Odile ;

Malanga, Maria ;

Boukamp, Petra ;

Althaus, Felix ;

Buerkle, Alexander .

NUCLEIC ACIDS RESEARCH, 2008, 36 (19) :6309-6317

[4] PARP is activated at stalled forks to mediate Mre11-dependent replication restart and recombination [J].

Bryant, Helen E. ;

Petermann, Eva ;

Schultz, Niklas ;

Jemth, Ann-Sofie ;

Loseva, Olga ;

Issaeva, Natalia ;

Johansson, Fredrik ;

Fernandez, Serena ;

McGlynn, Peter ;

Helleday, Thomas .

EMBO JOURNAL, 2009, 28 (17) :2601-2615

[5] Combining affinity purification by ADP-ribose-binding macro domains with mass spectrometry to define the mammalian ADP-ribosyl proteome [J].

Dania, Nadia ;

Stilla, Annalisa ;

Marchegiani, Adriano ;

Tamburro, Antonio ;

Till, Susanne ;

Ladurner, Andreas G. ;

Corda, Daniela ;

Di Girolamo, Maria .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (11) :4243-4248

[6] Functional association of poly(ADP-ribose) polymerase with DNA polymerase α-primase complex:: A link between DNA strand break detection and DNA replication [J].

Dantzer, F ;

Nasheuer, HP ;

Vonesch, JL ;

de Murcia, G ;

Menissier-De Murcia, J .

NUCLEIC ACIDS RESEARCH, 1998, 26 (08) :1891-1898

[7] The NoRC complex mediates the heterochromatin formation and stability of silent rRNA genes and centromeric repeats [J].

Guetg, Claudio ;

Lienemann, Philipp ;

Sirri, Valentina ;

Grummt, Ingrid ;

Hernandez-Verdun, Daniele ;

Hottiger, Michael O. ;

Fussenegger, Martin ;

Santoro, Raffaella .

EMBO JOURNAL, 2010, 29 (13) :2135-2146

[8] The diverse biological roles of mammalian PARPS, a small but powerful family of poly-ADP-ribose polymerases [J].

Hassa, Paul O. ;

Hottiger, Michael O. .

FRONTIERS IN BIOSCIENCE-LANDMARK, 2008, 13 :3046-3082

[9] The functional role of poly(ADP-ribose)polymerase 1 as novel coactivator of NF-κB in inflammatory disorders [J].

Hassa, PO ;

Hottiger, MO .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2002, 59 (09) :1534-1553

[10] Toward a unified nomenclature for mammalian ADP-ribosyltransferases [J].

Hottiger, Michael O. ;

Hassa, Paul O. ;

Luescher, Bernhard ;

Schueler, Herwig ;

Koch-Nolte, Friedrich .

TRENDS IN BIOCHEMICAL SCIENCES, 2010, 35 (04) :208-219

← 1 2 3 4 5 6 7 →