Pancreatic lipase inhibitory constituents from Morus alba leaves and optimization for extraction conditions

被引：53

作者：

Jeong, Ji Yeon ^{[1
]}

Jo, Yang Hee ^{[1
]}

Kim, Seon Beom ^{[1
]}

Liu, Qing ^{[1
]}

Lee, Jin Woo ^{[1
]}

Mo, Eun Jin ^{[1
]}

Lee, Ki Yong ^{[2
]}

Hwang, Bang Yeon ^{[1
]}

Lee, Mi Kyeong ^{[1
]}

机构：

[1] Chungbuk Natl Univ, Coll Pharm, Cheongju 361763, Chungbuk, South Korea

[2] Korea Univ, Coll Pharm, Sejong City 339700, South Korea

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2015年 / 25卷 / 11期

基金：

新加坡国家研究基金会;

关键词：

Morus alba; Optimization; Pancreatic lipase; Phenolic compounds; Response surface methodology; RESPONSE-SURFACE METHODOLOGY; 2-ARYLBENZOFURAN DERIVATIVES; FLAVONOIDS; TYROSINASE; HYDROXY; BARKS; LEAF;

D O I：

10.1016/j.bmcl.2015.04.045

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The leaves of Morus alba (Moraceae) have been traditionally used for the treatment of metabolic diseases including diabetes and hyperlipidemia. Thus, inhibitory effect of M. alba leaves on pancreatic lipase and their active constituents were investigated in this study. Twenty phenolic compounds including ten flavonoids, eight benzofurans, one stilbene and one chalcones were isolated from the leaves of M. alba. Among the isolated compounds, morachalcone A (20) exerted strong pancreatic lipase inhibition with IC50 value of 6.2 mu M. Other phenolic compounds containing a prenyl group showed moderate pancreatic lipase inhibition with IC50 value of <50 mu M. Next, extraction conditions with maximum pancreatic lipase inhibition and phenolic content were optimized using response surface methodology with three-level-three-factor Box-Behnken design. Our results suggested the optimized extraction condition for maximum pancreatic lipase inhibition and phenolic content as ethanol concentration of 74.9%; temperature 57.4 degrees C and sample/solvent ratio, 1/10. The pancreatic lipase inhibition and total phenolic content under optimized condition were found to be 58.5% and 26.2 mu g GAE (gallic acid equivalent)/mg extract, respectively, which were well matched with the predicted value. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：2269 / 2274

页数：6

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